LJH-OS人骨肉瘤裸鼠原位移植模型的建立及其生物学特性

Establishment and Biological Character of an Orthotopic Transplantation Model of Osteosarcoma with the LJH-OS Carcinoma Cell

用人成骨肉瘤细胞系LJH- OS的传代移植瘤组织作为移植材料,进行胫骨原位移植及皮下移植。结果发现胫骨原位移植的潜伏期较短,生长快。皮下移植瘤呈局限性膨胀性生长,有不完整的纤维包膜,未见肺转移,观察7 周无明显消瘦;而胫骨原位移植瘤浸润基层,无纤维包膜,且发生肺转移,7 周时有明显消瘦。原位移植的裸鼠血清ALP水平高于皮下移植者。说明裸鼠胫骨微环境较皮下组织更适于人骨肉瘤的浸润及转移表达,裸鼠胫骨原位移植模型的恶性生物学行为更接近临床骨肉瘤患者的实际情况,该原位移植模型的建立为骨肉瘤的研究提供了良好的实验模型。

Cell suspensions of LJH-OS were injected to nude mice subcutaneous. In tact tumor pieces, obtained from the third serial passage of subcutaneously growing xenografts, were implanted into the proximal tibia and the subcutaneous tissues of nude mice respetively. Animals were sacrificed and autopsied 4 and 7 weeks after transplantation and examined macroscopically for local tumor growth and metastasis. The tumor formation rate, the growth rate, the invasion and metastatic behavior of the orthotopic and ectopic xenografts were investigated and compared. We discovered the tumor with orthotopic transplatation had a faster growth rate than the s.c implantation. Tumors in the tibia invaded and destroyed the normal structure of the tibia bone, also infiltrated to the nearby tissues. The lung metastasis and reactive bone formation were observed only in the tibia transplatation model. It can be concluded the orthotopic transplantation of tumor tissue of the LJH-OS cell line into the tibia of nude mice resulted in local tumor growth and spontaneous metastasis which closely resembled the clinical situation. Therefore this model may be useful for experimental therapy studies and for investigations on the role of bone in regulating tumor growth, invasion and metastasis of osteosarcoma.