合肥地区献血员庚型肝炎病毒的分子生物学研究

A study on GBV-C/hepatitis G virus infection in blood donors in Hefei

目的 了解合肥地区献血员的庚型肝炎病毒(GBV-C/HGV)感染情况。方法 应用酶免疫测定法(EIA)和逆转录一套式多聚酶链反应法(RT-PCR)分别检测献血员中的抗-GBV-C/HGV和GBV-C/HGV RNA。结果 献血员中抗-GBV-C/HGV检出率为1.7％(18/1050),抗-GBV-C/HGV阳性血清中GBV-C/HGV RNA占66.7％(12/18);男性和低年龄组献血员抗-GBV-C/HGV阳性率分别低于女性和高年龄组,两者差异有显著性(P<0.05);抗-GBV-C/HGV阴性的献血员有6名转为阳性,2名抗-GBV-C/HGV和GBV-C/HGV RNA阳性的献血员有1名转为阴性。结论 GBV-C/HGV在合肥地区献血员中有较高的感染率;献血员中存在着GBV-C/HGV阳性但ALT正常的献血员,应尽快对献血员进行GBV-C/HGV感染指标的检测。

Objective To study the prevalence of GBV-C/HGV infection in blood donors in Hefei. Methods Plasma antibody against GBV-C/HGV( anti-GBV-C/HGV) and GBV-C/HGVRNA were detected with enzyme im-munoassay(EIA) and reverse transcription nested polymerase chain reaction(RT-nPCR) in blood donors. Results Posi-tivity of anti-GBV-C/HGV was 1.7 percent in 1 050 blood donors (18/1 050), and that of GBV-C/HGV RNA was 66.7 percent in those with positive anti-GBV-C/HGV( 12/18) .The positive rate of anti-GBV-C/HGV in male or low age group was higher than in female or high age group respectively , they had donors' ALT levels. Followed-up 200 blood donors for three months, six blood donors was converted into positivity (6/198), one blood donors with positive anti-GBV-C/HGV and GBV-C/HGV RNA was converted into negative. Conclusion It suggests that infection with GBV-C/HGV is more often in blood donors in Hefei. There were blood donors with positive GBV-C/HGV but normal ALT activities. It is ur gent to detect the markers of GBV-C/HGV infection in blood donors as soon as possible.