原发性肺癌bcl-2和p16蛋白表达与肿瘤临床病理学特征及预后的关系

Study of bcl-2 and p16 protein expression in lung cancers and their relationship to clinical course and progno-sis

目的:观察凋亡抑制基因bcl-2和多肿瘤抑制基因P16在原发性肺癌组织中的表达,探讨其与肺癌发生、发展的关系。方法:采用即用型SABC免疫组织化学方法观察bcl-2和p16蛋白在63例肺癌组织中的表达情况。结果:63例肺癌bcl-2和p16蛋白阳性表达率分别为50.8％和55.6％;随着肿瘤分化程度的降低,bcl-2和p16蛋白的阳性表达率下降,高分化和低分化肺癌之间bcl-2和p16阳性表达率的差异有显著性(P<0.05);Ⅰ～Ⅱ期与Ⅲ～Ⅳ期肺癌之间bcl-2和p16阳性表达率的差异亦有显著性(P<0.05);而且bcl-2和p16阳性表达率与肺癌预后显著相关(P<0.05);bcl-2蛋白表达与p16蛋白表达呈正相关。结论:bcl-2和p16蛋白高表达是原发性肺癌的早期表现,与肺癌发生、发展密切相关,可作为反映肺癌生物学行为和预后的重要标记物。

Purpose:To examine the expessions of bcl-2 and p16 proteins in lung cancers and to detemine the relatlonship be-tween the gene and lung cancer. Method:Using SABC method of immunohistochemical staining, we examined the samples of 63 patients. Results: The positive rates of bcl-2 and p16 proteins were 50. 8% and 55. 6% respectively in lung cancers; The positive rates of bcl-2 and p16 proteins were significantly higher in the patients with good-differentiation, grade I of histological grading and without metastasis than that in patients with poor-differentiation, gradeⅢof histological grading and with metastasis (P< 0. 05); The positive rates of bcl-2 and p16 proteins were correlated with the TNM staging and with the prognosis (P<0. 05); positive correlation was found between the expression of bcl-2 and p16 proteins. Conclusions: The expressions of bcl-2 and p16 proteins mayt be the important markers reflecting the biological behaviors and prognosis of lung cancers.