胃癌巨噬细胞浸润和微血管密度与侵袭转移的关系

RELATIONSHIP BETWEEN MACROPHAGE INFILTRATION AND MVD IN GASTRIC CARCINOMA

目的:探讨胃癌组织中浸润巨噬细胞与微血管密度的相关性及其在胃癌侵袭转移中的作用。方法:应用免疫组化SP法,用抗CD68 和抗CD34单克隆抗体对102例胃癌组织进行标记分析,并应用计算机辅助图像分析系统对血管新生的面积进行定量分析。结果:巨噬细胞浸润与胃癌分化程度、浸润深度、淋巴结转移和TNM分期有关,且有显著性差异(p<0.01,p<0.05,p<0.05, p<0.05)。MVD与分化程度、浸润深度、淋巴结转移,TNM分期相关,且有显著性差异(p<0.01)。巨噬细胞计数与MVD呈正相关(r=0.961,p<0.01)。结论:联合检测巨噬细胞浸润度及MVD定量分析可提供胃癌恶性转归更有价值的指标。

Objective:To investigate the correlation of macrophages and MVD in gastric carcinoma and the effect on invasion and metastasis. Method:The expression of macrophages and MVD in 102 cases of gastric carcinoma were investigated by immunohistochemical SP method using monoclonal antibody to CD68 and CD34. The area of neovascularization was assessed quantitatively by computer-assisted video analysis system. Results: There were relativity and significant differences between macrophage counts and differentiation,depth of invasion,lymph node metastasis and TNM stage (p<0.01,p<0.05,p<0.05,p<0.05). There were relativity and significant differences between MVD and differentiation,depth of invasion,lymph node metastasis and TNM stage ( p<0.01). A positive correlation was found between MVD and macrophage counts (r=0.961,p<0.01). Conclusion: Combining examinations of macrophages and MVD will be a better predictor for malignant degree in gastric carcinoma .