甲酰四氢叶酸钙调变5-氟脲嘧啶对胃癌原代细胞株增殖抑制效应

Regulatory Effect of CF on 5-FU Against Human Gastric Cancer Cell Line in Vitro

目的：探讨甲酰四氢叶酸钙（ＣＦ）调变５－氟脲嘧啶（５－ＦＵ）对不同胃癌原代细胞株的增殖抑制效应。方法：采用原代细胞培养和四唑氮蓝（ＭＴＴ）药物敏感试验，观察大剂量ＣＦ相当于临床用药３００ｍｇ／ｄ）和５－ＦＵ（相当于临床用药７５０ｍｇ／ｄ）单剂和联合应用对胃癌原代细胞的体外抑制作用。结果：体外对胃癌原代细胞株直接抑制作用，单剂ＣＦ为１１．８２％，单剂５－ＦＵ为４２．２５％；两者联合应用为４４．５４％，仅４３％的胃癌原代细胞株显示ＣＦ对５－ＦＵ有一定的增效作用。结论：单剂ＣＦ对胃癌原代细胞仅有轻度抑制作用，联合应用大剂量ＣＦ和５－ＦＵ仅显示ＣＦ对部分细胞株５－ＦＵ的抑制作用有一定的增强效应，个体差异性较大，为确保ＣＦ的生化调节作用，避免其大剂量使用时毒副作用，建议用药前行ＭＴＴ法药敏试验，以指导临床制订合理联合应用ＣＦ和５－ＦＵ的化疗方案。

Background/Aims: To evaluate the regulatory effect of calcium folinate (CF) on 5-fluorouracil (5-FU) against human gastric cancer cell. Methods: Primary cell culture technique and MTT colorimetric assay were used to study propagational inhibition effect of CF, 5-FU and a combination of the two drugs on 30 fresh human gastric cancer cell lines in vitro. Results: The immediate propagational inhibition rates of CF, 5-FU and CF/5-FU against gastric cell lines were 11.82%, 42.25% and 44.54% respectively. The combination of the two drugs had a slight rise in propagational inhibition rate in 43% of gastric cancer cell lines. Conclusions: CF increased the anticancer effect of 5-FU in small part of gastric cancer cell lines in vitro. The drug sensitivity test by MTT should be performed before chemotherapy using CF and 5-FU.