结直肠癌部位与p53基因突变的关系

Relationship between p53 Gene Mutation and Location of Colorectal Carcinomas

进一步阐明p53外显子突变率与结．直肠癌不同部位之间的关系。方法：选取108例大肠癌患者癌灶组织，用PCR-SSCP法进行p53第5～8外显子的检测。结果：108,例大肠癌标本中，53例发生p53基因突变，突变率为49．07％(53／108)，统计学处理结果表明，外显子与大肠癌部位有显著关系(X^2=73．683，P<o．001)。结论：不同部位的太肠癌患者其p弱基因第5～8外显子的突变率不同，筛选最有效的外显子进行检测，可有效地提高检测效率，降低检测成本，减轻病员负担，并在临床推广应用中起积极作用。

Background/Aims: To explore the relationship between exon mutation rate of p53 gene and various locations of colorectal cancer. Methods: 108 cases of colorectal cancer underwent surgical operation. PCR～SSCP was used to detect p53 gene exon 5～8 mutation from surgical specimens. Results: 53 of 108 patients had p53 gene mutation, the mutation rate was 49.07% (53/108). There was statistically significant difference between the various locations of colorectal cancer and the p53 exon mutation (X2=73.683, P<0.001). Conclusions: There are different p53 exon mutation rates in various locations of colorectal cancer patients. Highly～selected and mutated exons to detect the gene mutation may increase the detection rate and lower the costs, and can be used clinically.