炎症性肠病患者树突状细胞的免疫功能研究

Study on the Immunologic Function of Dendritic Cells from Peripheral Blood in Patients with Inflammatory Bowel Disease

研究炎症性肠病（ＩＢＤ）患者外周血树突状细胞（ＤＣ）的免疫功能，探讨ＤＣ免疫功能变化在ＩＢＤ发生中的作用。方法：以健康成人（ｎ＝１０）作为对照，自ＩＢＤ患者（ｎ＝１１）外周血中分离和纯化ＤＣ及Ｔ细胞，按间接免疫荧光法通过流式细胞仪检测ＤＣ表面ＨＬＡ－ＤＲ及Ｂ７－１的表达水平，检测ＤＣ诱导自体混合Ｔ淋巴细胞增殖反应的能力。结果：ＩＢＤ患者 ＤＣ表面 ＨＬＡ－ＤＲ、 Ｂ７－１的表达水平及 ＤＣ诱导自体混合 Ｔ淋巴细胞增殖的能力（１９．６ＶＯＦ±１．３ＶＯＦ，１８．７ＶＯＦ±１．６ＶＯＦ，１２３１０ｃｐｍ±１４６ｃｐｍ）明显高于对照组（１３．６ＶＯＦ±１．２ＶＯＦ，１２．８ＶＯＦ±１．４ＶＯＦ，Ｐ＜０．０５，８６６０ｃｐｍ±１２０ｃｐｍ，Ｐ＜０．０１）。结论： ＩＢＤ患者ＤＣ免疫功能亢进。提示ＤＣ免疫功能亢进可能与ＩＢＤ的发生及发展相关。

Background/Aims: To study the immunologic function of dendritic cells (DC) from peripheral blood in patients with inflammatory bowel disease (IBD) and evaluate the relation of the immunologic function of DC to IBD. Methods: DC was isolated and purified from peripheral blood of IBD patients (n=11) and compared with healthy adults (n=10) to detect the expression levels of HLA-DR and B7-1 costimulatory molecule on DC and evaluate the ability of DC inducing mixed T lymphocytes prolifera- tion. Results: The expression levels of HLA-DR and B7-1 and the ability of DC inducing mixed T lym- phocytes proliferation (19.6VOF±1.3VOF, 18.7VOF±1.6VOF, 12310cpm±146cpm) in IBD were higher than those in the control group (13.6VOF±1.2VOF,12.8VOF±1.4VOF, P<0.05, 8660cpm±120cpm, P<0.01). Conclusions: The expressions of HLA-DR and B7-1 on DC and the immunologic function of DC in IBD are up-regulated, which are closely related to IBD.