不同注射途径对糖化多聚赖氨酸偶联的表达质粒在大鼠肝脏靶向分布表达的影响

Comparative Studies of Different Introducing Routes on Liver Targeted Uptake and Gene Expression of Plasmid Delivered by Glyco-poly-L-lysine

比较静脉注射途径和腹腔注射途径对糖化多聚赖氨酸（Ｇ－ＰＬＬ）偶联的目的基因在肝脏靶向分布表达的影响。方法：将真核细胞表达质粒与Ｇ－ＰＬＬ偶联成复合物，分别经尾静脉和腹腔注射导入大鼠体内，通过原位杂交和免疫组化法，观察目的基因在肝脏和其他脏器的分布表达。结果：运用Ｇ－ＰＬＬ偶联的质粒ＤＮＡ经两种途径导入体内２４小时后均见明显表达，１周后逐渐下降，３周时仍有表达；均以肝脏为主要分布器官，但经静脉途径导入者，质粒ＤＮＡ在肝脏中的分布表达高于经腹腔途径导人者。结论：经静脉途径导人的Ｇ－ＰＬＬ／质粒ＤＮＡ复合物在肝脏的靶向定位效果优于经腹腔途径导入者。

Background/Aims:To compare the effects of intravenous route and peritoneal route on liver tar- geted uptake and gene expression of the plasmid pTM/MMP-1 delivered by glyco-poly-L-lysine (G-PLL). Methods: The plasmid which could be expressed in eukaryotic cells was bound to the galac- tose-terminal G-PLL and was then transferred into rat's body by intravenous injection and imtraperi- toneal injection, respectively, then the results were observed in different periods by in situ hybridiza- tion and immunohistochemistry. Results: The expression of the plasmid binding to G-PLL was obvious 24 hours later after the plasmid had been transferred in vivo through intravenous route and intraperi- toneal route, and the expression began to decrease one week later, but could still be found three weeks later. Both intravenous route and intraperitoneal route made the liver as the major distribution and expression tissue of the plasmid, but the distribution and expression of the plasmid transferred via intravenous route were higher in liver than that transferred via intraperitoneal route. Conclusions: The effects of intravenous route on liver target uptake and expression of the plasmid binding to G-PLL are better than that of peritoneal route.