缺血预处置对大鼠分离的心肌细胞缺氧/再给氧时腺苷酸的影响

Effect of in Chemia precondition on the adenylic acid in isolated myocytes during ischemia/reperfusion in rats

以生命的基本单位—细胞为模型进行研究者国内比较少见,本实验选用雄性Wistar大鼠体重250～300g,采用改进的Farmer法分离心肌细胞,然后将游离的心肌细胞分为6组:正常对照组(N)、缺氧20分钟组(I20′)、缺氧20分钟再给氧10分钟组(I20′R10)、缺氧40分钟组(I40′)、缺氧40分钟再给氧10分钟组(I40′R10′)、预处置组(PC)。用高效液相色谱法测定心肌细胞内腺苷酸(ATP、ADP、AMP)的含量。实验结果显示:随着缺氧时间的延长,心肌细胞内ATP的含量逐渐下降;早期缺氧/再给氧时(I20′R10′),ATP含量回升,晚期(140′R10′)则明显下降;预处置组较未经预处置组(I40′R10′)的ATP含量明显增高。上述结果说明,缺氧20分钟再给氧10分钟,损伤为可逆性的;缺氧40分钟再给氧10分钟,损伤为不可逆性的,即发生了较严重的再灌注损伤。预处置对缺氧心肌具有一定的保护作用。

It is very rare that cell-hase uite of life wasstudied in civie.Wistar adult rats, male 250~300gwereused in the experiment and the improved palmer's method was adopted to isolate myocytes. isolated myoeytes were divided randomly into 6 groups, nomally control (N) . ischemia 20 min (I20') , ischemia 20 min/reperfution 10min (I 20'R10') , ischemia 40 min (I40') , ischemia 40min/reperfusion 10 min (I40'R10') . and ischemiaprecondionig. We detected the contents of adenylicacid (ATP, ADP, AMP) by using High-performance Liguidchromtograph. The results showed that the level of ATP of myocytesdecrease gradually with the prolongation of hypoxia. Inan early stage of ischemia/reperf usion (I20'/R10') . thecontents of ATP was increased, but ito bviouslydoecreases at a later stage (I40'R10') . The content ofATP in Preconditiong group (I40'R10') were obviouslyincreased compared with one of untreated. The results suggest that the injury is reAersibledurng the perid of ischemia 20 min/re-pferusion 10 min, the irrerversible are comes aboutthe 10 minreoxyention following 40 min hypoxia, the seriousreperfusion injury. The protective effect of preconditioniing on myocardium.