7种喹诺酮类药物固体表面室温磷光或延迟荧光分析法研究

Study on the Solid Surface Room Temperature Phosphorimetry or Delayed Fluorescence of Seven Quinolones

建立了滤纸为基质的 7种喹诺酮类药物的固体表面室温磷光 ( SS- RTP)或延迟荧光 ( SS- DF)分析测定的新方法 .对影响喹诺酮类药物发光强度的各种因素 ,包括介质 p H条件、重原子种类、浓度等进行了详细的研究 .实验表明 ,除氧氟沙星在 p H=6 .5条件下有较强室温磷光发射外 ,其余 6种药物均在酸性条件下有较强 RTP或 DF发射 ,其适宜 p H为 1.6或 1.8.方法的稳定性好 ,线性范围宽 ,检出限低 .7种喹诺酮类药物在尿样中的回收率最大为 10 4.3% ,最小为 97.7% 。

The solid surface room temperature phosphorimetry (SS\|RTP) or the solid surface delayed fluorescence (SS\|DF) of seven kinds of quinolones, ciprofloxacin, fleroxacin, lomefloxacin, ofloxacin and pefloxacin, norfloxacin,pipemidic were first established with filter paper as a solid substrate.Various effect factors on SS\|RTP or SS\|DF intensity such as substrate,pH condition,selection of heavy ions and inference of their concentration,drying condition and the stability were studied in detail.The linear range, the detection limit and RSD for every kind of above quinolones were described and the discovery the pharmaceutical analysis results are satisfactory.