十一酸睾酮对雄性骨质疏松大鼠骨代谢的影响

Effect of andriol on the bone metabolism of male rats with osteoporosis

目的:研究十一酸睾酮替代治疗对雄性去势骨质疏松大鼠骨代谢的影响,以期为男性骨质疏松症的预防提供思路。方法:实验选用15周龄雄性SD大鼠32只去睾后作为动物模型,随机分为对照组11只、模型组11只、治疗组10只,28周后行血尿生化、骨密度检查。结果:模型组大鼠全身骨密度、股骨中点骨密度、睾酮水平犤(0.351±0.012),(0.357±0.007)g/cm2,(0.01±0.02)nmol/L犦明显低于对照组犤(0.366±0.021),(0.366±0.012)g/cm2,(1.82±0.02)nmol/L犦(P<0.05～0.01)。治疗组大鼠全身骨密度、股骨中点骨密度、睾酮水平、雌二醇水平明显高于模型组(P<0.05～0.01)。模型组大鼠尿钙/肌酐、尿磷/肌酐、尿羟脯氨酸/肌酐水平明显高于对照组(P<0.05～0.01)。治疗组大鼠血碱性磷酸酶水平明显高于模型组(P<0.05)。结论:早期应用十一酸睾酮替代治疗可逆转雄性大鼠去睾28周后骨质疏松的发生。

AIM:To study the influence of replacement therapy of andriol on the bone mechanism of male castrated rats with osteoporosis,so as to provide idea for the prevention of male osteoporosis. METHODS:Thirty-two 15-week-old male SD rats were castrated as the models.Experimental animals were randomly divided into 3 groups: control group(n=11),model group(n=11) and treatment group(n=10).After 28 weeks, blood urine biochemical indexes and bone mineral density(BMD) were performed. RESULTS:The whole body BMD,BMD at the middle point of femur and the level of testosterone were obviously lower in the model group [(0.351 ±0.012), (0.357±0.007) g/cm2, (0.01±0.02) nmol/L]than in the control group[(0.366±0.021),(0.366±0.012) g/cm2,(1.82 ±0.02) nmol/L] (P< 0.05 to 0.01);The above-mentioned indexes and the level of estradiol were obviously higher in the treatment group than in the model group(P< 0.05 to 0.01).The levels of urinary calcium/creatinine(Cr),urinary phosphorus/Cr and urinary hydroxyproline/Cr in the model group were obviously higher than those in the control group(P< 0.05 to 0.01). The level of blood alkaline phosphatase in the treatment group was obviously higher than that in the model group(P< 0.05). CONCLUSION:Early application of the replacement therapy of andriol can prevent the occurrence of osteoporosis in male rats 28 weeks after castration.