心肌氧化应激损伤中硫氧还蛋白的作用

Role of thioredoxin in myocardial oxidative-stress injury

目的:探讨硫氧还蛋白(thioredoxin,TRX)在心肌氧化应激损伤中的作用并分析其机制。方法:本研究选用Wistar大鼠16只,体质量180～200g,随机分为2组:冰泳负荷组和对照组,每组8只。让冰泳负荷组大鼠在冰水浴中泳动5min,用透射电镜观察心肌损伤情况,采用northernblot和westernblot杂交方法检测心肌组织中硫氧还蛋白的蛋白和基因表达变化。结果:我们透射电镜下观察到,冰泳负荷组大鼠心肌细胞及线粒体受到损伤,对照组心肌细胞未发生异常改变。northernblot和westernblot杂交结果显示,冰泳负荷组大鼠心肌TRX基因水平较对照组升高,同时冰泳负荷组大鼠心肌TRX蛋白含量也比对照组增加。结论:TRX蛋白在心肌的氧化应激防御反应中起重要作用。

AIM: To study the role of thioredoxin(TRX) in the oxidative-stress injury of myocardium and analyze its mechanism. METHODS: Sixteen Wistar rats, weighing 180 to 200 g, were randomly divided to two groups with 8 rats in each group: ice-swim stress group and control group. The myocardial injury of the rats in the ice-swim stress group was observed with transmission microscope after swam in the ice-water bath for 5 minutes. The changes of TRX protein and gene expression of in myocardium were examined with western blot and northern blot. RESULTS: Under the transmission microscope, injuries of myocardium and mitochondria in the ice-swim stress group were observed, while there was no abnormal change of myocardium in the control group. The results of western blot and northern blot showed that the level of TRX gene and contents of TRX protein in the ice-swim stress group were increased as compared with those in the control group. CONCLUSION:TRX protein plays an important role in the defense response of myocardium against oxidative stress.