细胞外温度变化及降温速度和程度对细胞损伤的实时监测研究

Extracellular temperature changes and temperatuer-lowering speed and degree in metering real time of cell injury

目的:制作适合细胞培养时检测细胞温度的测温装置,通过试验确定其测温精度和检测其测温时间特性,为研究冻伤时细胞外温度变化提供技术条件。方法:实验于2003-12/2004-08在第四军医大学烧伤科实验室完成。试验中,采用DY2000-智能数字显示仪表和铂热电阻温度传感器两连接组成下位机系统,上位机程序由VisualBasic6.0(简称VB6)开发的可以用于windows2000professional操作系统平台的串口程序组成,然后将采集到的数据和具有国家二级标准的温度计读到的数据相比较。结果:自行研制的仪器用于细胞低温试验是一种体积小、精度高(0.01℃)、测温范围广(-70～100℃)、采样快(大于1000Hz的采样率),能够在毫秒级别智能记录试验结果的铂热电阻测温仪器。结论:自行研制的低温测温仪器适用于低温处理细胞培养时的测温。

AIM:To develop a temperature-measuring instrument suitable for temperature measurement during cell culture to confirm temperature-measuring precision and detect its time characters of temperature measurement through test so as to provide technical conditions for studying the extracellular temperature changes after frostbite. METHODS:The experiment was conducted in the laboratory of Department of Burns,Fourth Military Medical University of Chinese PLA. Lower processor was composed by connecting intelligent digital display instrument and Pt-resistance temperature sensor.Upper processor was composed of serial programs in windows 2000 professional system platform developed by Visual Basic 6.0(VB6).By using the lower and upper processor,the data were collected and the data recorded by thermometer at second national grade were compared. RESULTS:The self-developed instrument used in cell low temperature experiment with the characters of small volume,high precision(0.01 ℃),large measuring range(-70 ℃to 100 ℃) and rapid sampling(more than 1 000 Hz),was a Pt-resistance temperature meter that could record experimental results intelligently at millisecond level. CONCLUSION:The self-developed instrument is fit for metering the extracellular temperature of cell culture at low temperature.