山莨菪碱抗肺缺血再灌注损伤作用的实验研究

Experimental study on protective effect of anisodamine on lung ischemia-reperfusion injury in rabbits

目的 探讨山莨菪碱 (6 5 4 - 2 )抗兔肺缺血再灌注损伤作用及其机制。方法 在建立兔单肺原位缺血再灌注损伤模型上 ,观察山莨菪碱对肺组织湿 /干比值 (W /D)、丙二醛 (MDA)、肺匀浆髓过氧化物酶(MPO)活性和肺毛细血管通透性 (LPI)作用和肺组织光镜、电镜形态学变化肺组织损伤 (LTD)程度。结果 缺血 90min、再灌注 12 0min后 ,肺组织的W /D、LPI、MDA、MPO活性、LTD程度减少 ,6 5 4— 2可使肺组织病理损伤明显减轻。结论 山莨菪碱具有抗肺缺血再灌注损伤作用 ,其作用机制可能与抑制中性粒细胞在肺内聚集 ,减轻氧自由基造成的肺损伤有关。

Objective To explore the protective effects of anisodamine(654-2) on lung and its possible mechanism during lung ischemia-reperfusion injury.Methods Single lung in situ warm ischemia reperfusion rabbit model was set up. Thirty Japanese rabbits were randomly divided into three equal groups, the control group, the IR group,and the ischemia-reperfusion group with anisodamine (8mg/kg) intravenous injection. The changes of lung tissue wet weight/dry weight ratio(W/D), malondialdehyda(MDA) and myeloperoxidase(MPO)activity, as well as the lung permeability index(LPI) were checked in each group. The histological appearances of the lungs were observed under histology and electron microscopy. Some sections were examined as an index of quantitative assessment of the degree of lung tissue damage(LTD).Results After 90 min ischemia and 120 min reperfusion, W/D,MDA, LPI, MPO activity, and LTD degree were significantly higher in IR group than those of the control group (P<0.01).Anisodamine was effective in reducing W/D, MDA, LPI,MPO activity and LTD(P<0.01). Histological examination of lung tissues demonstrated that the degree of lung injury had been alleviated significantly.Conclusion The lung tissue ischemia-reperfusion injury can be significantly protected with anisodamine. The protective mechanism may be that anisodamine can effectively reduce the neutrophil sequestration within the lung and the lung injury resulting from oxygen-derived free radical.