极端嗜热纤维素分解菌分子特征

Molecular Characteristic of Thermoaphilic Cellulolytic Anaerobic Bacteria

从云南邦拿掌温泉中采集样品,经过多次富集、筛选,最后分离出6株高温严格厌氧纤维素分解细菌.通过随机扩增多态性DNA(RAPD)分析,研究了这6株形态、生理、代谢特性相似的高温严格厌氧纤维素分解菌的遗传多样性,并根据实验统计数据作出6株菌的亲缘关系图.同时对产酶活性较高的B7细菌进行16SrDNA序列分析,确定了其系统分类地位.

Collection the specimen was isolated from Bang Na Zhang thermal spring,Yun Nan province Several strict thermophilic celluloytic anaerobic bacteria was isolated after plentful enrichment and filtrationThe genetic diversity of these bacteria,which the characters of their morpha、phsiological propetry and metabolism were resemblant,was decided by doing the experiment of random amplified polymorphic DNA ((RAPD))The genetic relation of several stains of Bang was done with statistical data of RAPDThe phylogenetic analysis based on 16S rDNA suggest strain B7 one of these bacteria which cellulose was more peak and stabilization