铅对离体人胎盘绒毛毒性作用的研究

EFFECTS OF LEAD ON HUMAN PLACENTAL CHORION IN VITRO

选用人流所得胎盘绒毛,分别用组织块法和器官法进行离体培养铅毒实验。组织块法加铅培养3天后,器官法加铅培养4天后,分别进行普通光镜、扫描电镜和透射电镜观察。同时,用放免分析的方法测定器官法培液中培养96小时后的HCG的累积含量。结果表明,当培液中的铅含量达2500μg/L时,无论是组织块法还是器官法培养的人胎盘绒毛均表现出中毒的形态学改变。培液中的HCG96小时累积含量亦明显低于对照组。说明铅对胎盘具有直接的毒性作用,但产生中毒现象时的铅浓度明显高于动物载体实验。由此分析,铅对载体胎盘的毒性作用是综合性的,直接毒性作用仅是其中的一个方面。铅对胎盘的毒性作用无疑是铅胚胎毒机理中的重要一环。

Fresh human placental chorion were cultured together with different concentration of lead in explant, monolayer culture and organ culture respectively. The morphology was examined under the light and electron microscope(S. E. M and T. E. M), and HCG was radioimmuoassayed in the culture medium on the 3rd day inexplant, monolayer culture and on the 4th day in organ culture. The results showed that when the level of lead in the medium reach 2500μg/L, the toxic effects of lead on human placental chorion emerged. As lead level increased, the toxic effects became more obvious. The level of HCG after culturing for 96 hours in mediums with lead at 2500μg/L and 5000μg/L were significantly lower than that in the control group (P<0.01). It is indicated that lead had directly toxic effects on human placental chorion in vitro. However, the concentration of lead inducing observed toxic effect was higher than in vivo.