摘要
目的 观察一种新型组织相容性白细胞抗原(HLA)衍生肽(RDP1258)的免疫抑制作用并探讨其机制。方法 人工合成一种HLA RDP1258,用3H胸腺嘧啶( 3H TdR)掺入法观察其在体外对大鼠脾细胞增殖反应的影响;以酶化学法观察其对脾细胞血红素氧合酶 1(HO 1)活性的影响。并与HLA衍生肽(HLA 2702.75 84)进行对比。结果 HLA RDP1258 抑制大鼠脾细胞增殖作用比HLA B2702.75 84 强,两者比较,差异有统计学意义(P<0.05);HLA RDP1258 对淋巴细胞增殖具有显著的抑制作用,抑制率大约是 HLA B2702.75 84 的 8 倍以上;HLA RDP1258 具有较明显的体外HO 1抑制作用,是HLA B2702.75 84抑制作用的5倍以上,呈剂量依赖性。结论 HLA RDP1258能够显著抑制大鼠脾细胞经丝裂原或同种抗原刺激引起的增殖反应,这种作用可能与影响HO 1活性有关。
Objective To observe the immunosuppression function of a novel HLA derived peptide, RDP1258, and to investigate the mechanisms. Methods The peptides derived from HLA, RDP1258, and HLA 2702.75 84 was synthesized chemically. The effects of the peptides on alloreactive cytotoxic activities of rat spleen cells were observed using 3H TdR incorporation method. The heme oxygenase 1 activity was detected by the enzyme chemistry method. Result The synthetic HLA derived peptides could obviously inhibit the proliferation of rat spleen cells and HO 1 activity in a dose dependent manner in vitro. Conclusion HO 1 may participate in the process of inhibitory effect of RDP1258 on the proliferation of rat spleen cells induced by mitogen or isoantigen.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第2期81-83,共3页
Chinese Journal of Organ Transplantation
基金
国家新药基金资助项目(9690105184)
