摘要
目的 探讨白细胞介素11(IL 11)对急性淋巴细胞白血病(ALL)小鼠骨髓移植后移植物抗宿主病(GVHD)及移植物抗白血病(GVL)的影响及其可能的相关机制。方法 随机将 20 只裸鼠(免疫缺陷鼠)及20只BABL/c小鼠分别等分为 2 组,共计 4 组。A组:裸鼠对照组;B组:裸鼠实验组;C组:BABL/c小鼠对照组;D组:BABL/c小鼠实验组。实验组在移植前2 d至移植后7 d皮下注射 IL 11,对照组不注射 IL 11。用流式细胞术检测 IL 11对移植前后ALL小鼠 CD4+、CD8+ T细胞亚群的影响;记录ALL小鼠骨髓移植后的存活时间;观察移植后 ALL小鼠肝、肠、脾的 GVHD病理变化;ELISA检测肿瘤坏死因子α(TNF α)的水平。结果 D组在显著降低CD4+ T细胞亚群的同时,增加CD8+ T细胞亚群的数量;明显提高ALL小鼠的生存时间;病理观察发生 GVHD的时间推迟,且程度较轻。D组TNF α测定结果明显低于C组;D组与其他各组比较,差异均有统计学意义(P<0.01)。结论 ALL小鼠骨髓移植后皮下注射 IL 11,具有降低GVHD并且保留了GVL的作用。
Objective To observe the effect of IL 11 on graft versus host disease and graft versus leukemia after allogenic bone marrow transplantation and related mechanism in acute lymphoblastic leukemic (ALL) mice. Methods Twenty nude mice and 20 BABL/c mice were randomly divided into 2 groups separately, total 4 groups. Group A and C, nude mice and BABL/c mice control group respectively; Group B and D, nude mice and BABL/c mice experimental group. The mice in the experimental groups were subjected to the subcutaneous injection of IL 11 2 days before transplantation and 7 days after transplantation, while those in the control groups not to IL 11. The effects of IL 11 on CD4 + and CD8 + T cells before and after transplantation in ALL mice were evaluated by flow cytometry. The survival time of ALL mice after marrow transplantation was recorded. The GVHD pathological changes of liver, spleen and intestine in ALL mice after transplantation were observed. The level of tumor necrosis factor α (TNF α) was detected by ELISA. Results In group D, IL 11 could significantly decrease the number of CD4 + T cells and increase CD8 + T cells simultaneously. IL 11 could obviously prolong the survival time, delay and relieve GVHD, and decrease the level of TNF α in ALL mice after transplantation. Conclusion IL 11 could alleviate GVHD and retain GVL effect after allogenic bone marrow transplantation.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第2期84-86,共3页
Chinese Journal of Organ Transplantation
基金
湖北省卫生厅项目资助(WT97415)
