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超极化心脏保存液对离体大鼠心脏低温保存的保护作用

Protective effects of hyperpolarized cardiac preservation solution on isolated rat heart
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摘要  目的 探讨含K+通道开放剂的心脏保存液(HCS液)对大鼠离体心脏的保存作用。方法 将24只SD大鼠随机平均分为 3 组,分别用 HCS液、UW液、K H液对心脏进行保存。采用Langendorff心脏灌注和工作模型装置进行心脏功能测定,比较 24h后心功能恢复率以及细胞酶学、心肌含水量、pH值变化和能量变化,观察心肌超微结构改变。结果 心脏保存24 h后, K H组左心室功能损伤严重,UW组及HCS组左心室功能损伤较轻。HCS组冠状动脉流量(CF)恢复率较 K H组、UW组好;HCS组冠脉回流液中乳酸脱氢酶(LDH)、磷酸肌酸激酶(CPK)含量及心肌含水量与UW组比较,差异无统计学意义;HCS组 pH值变化最小, ATP含量最高;HCS组心脏复跳时间与UW组基本相同; HCS组心肌保存良好。结论 HCS保存液对大鼠心脏的保存在 24 h内有明显的保护作用,心脏功能恢复率能达到UW液的水平,在能量保护、改善酸中毒方面则优于UW液。 Objective To evaluate the protective effects of a novel hyperpolarized cardiac preservation solution on isolated rat heart. Methods Male Sprague Dawley rats (250~300 g, n =24) were randomly divided into three groups ( n =8 in each group) and their hearts were preserved with K H, UW, HCS solution respectively for 24 h. The isolated hearts were perfused in the Langendorff rat mode for functional evaluation before and after preservation. Lactate dehydrogenase and creatine phosphokinase enzyme leakages, contents of adenosine triphosphate and wet weights in myocardium were measured after reperfusion. Results The impairment of the left ventricle function in K H group was serious, while that in HCS and UW groups was mild. The recovery of coronary flow in HCS group was better than K H and UW groups. There were no significant differences in releases of LDH and CPK and wet weight of myocardium between UW group and HCS group. HCS group offered a less variety of pH and had the highest ATP content of myocardium among the three groups. The time, from the reperfusion to beating of heart, showed no difference between UW group and HCS group. The ultrastructural changes were slight in HCS group. Conclusions HCS solution can protect rat heart for 24 h effectively. The recovery of heart function by HCS is similar to that by UW solution. HCS solution can improve the coronary flow and acidosis and protect ATP more effectively than UW solution.
出处 《中华器官移植杂志》 CAS CSCD 北大核心 2005年第2期91-93,共3页 Chinese Journal of Organ Transplantation
基金 上海市科学技术委员会重点资助项目 (024919006)
关键词 超极化心脏保存液 离体大鼠心脏 低温保存 保护作用 Organ preservation Heart Rars
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参考文献7

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