MMP-2在新月体肾小球肾炎中的表达和作用

PROTEIN EXPRESSION AND EFFECT FOR MATRIX METALLOPROTEINASES-2 IN PATIENTS WITH CRESCENT GLOMERULONEPHRITIS

目的探讨MMP鄄2(matrixmetalloproteinases鄄2)在新月体肾小球肾炎中的表达和作用。方法1997～2002年间本院经肾活检证实新月体肾小球肾炎住院病人112例,其中细胞性新月体的肾活检标本60例为一组,纤维鄄细胞性和纤维性新月体52例为另一组。用免疫组化法测MMP鄄2和Ⅳ胶原蛋白表达并进行比较,并与10例正常肾组织对照。结果MMP鄄2在新月体中有强表达,细胞性新月体组MMP鄄2蛋白表达是正常肾组织的3.2倍,而纤维鄄细胞性和纤维性新月体组MMP鄄2蛋白表达比细胞性新月体组下降了52%,Ⅳ型胶原蛋白表达是细胞性新月体组的2.1倍。重复肾活检的40例新月体肾炎病人治疗后的肾组织MMP鄄2蛋白表达比治疗前下降了61%。结论新月体肾炎肾小球中过度表达MMP鄄2,早期治疗可抑制MMP鄄2表达,能及时挽救患者生命和改善预后。

OBJECTIVE: TO INVESTIGATE THE PROTEIN EXPRESSION AND EFFECT OF MATRIX METALLOPROTEINASES-2 IN PATIENTS WITH CRESCENT GLOMERULONEPHRITIS. METHODS: 112 PATIENTS WITH CRESCENT GLOMERULONEPHRITIS WERE COLLECTED IN 1997～2002, THESE RENAL BIOPSY SPECIMENTS OF 60 PATIENTS WITH CELL CRESCENT GLOMERULONEPHRITIS AS A GROUP,THESE RENAL BIOPSY SPECIMENTS OF 52 PATIENTS WITH FIBER-CELL AND FIBROSIS CRESCENT GLOMERRULONEPHRITIS AS ANOTHER GROUP.BOTH PROTEIN EXPRESSION OF MMP-2 AND Ⅳ COLLAGEN BY IMMUNOHISTOCHEMICAL STAINING WERE ANALYSIS, AND 10 HEALTHY RENAL SPECIMENTS AS CONTROL. RESULTS: THERE ARE STRONG MMP-2 PROTEIN EXPRESSION IN CRESCENT(3.2-FOLD INCREASE VS CONTROL, P < 0.001). MEANWHILE,WE FOUND THAT MMP-2 PROTEIN EXPRESSION IN FIBER-CELL AND FIBROSIS CRESCENT WERE OBVIOUSLY REDUCED(ABOUT 52% REDUCED, P < 0.05) THAN CELL CRESCENT,AND Ⅳ COLLAGEN PROTEIN EXPRESSION UPREGULATED IN FIBER-CELL AND FIBROSIS CRESCENT(AN ABOUT 2.1-FOLD INCREASE VS CELL CRESCENT). MMP-2 PROTEIN EXPRESSION IN RENAL TISSUE HAVE AN ABOUT 61% REDUCED AFTER TREATMENT FOR 40 PATIENTS OF CRESCENT GLOMERULONEPHRITIS WITH REPEATED RENAL BIOPSY. CONCLUSIONS: THERE ARE STRONG EXPRESSION OF MMP-2 IN CRESCENT GLOMERULONEPHRITIS. THESE PATIENTS WITH CRESCENT GLOMERULONEPHRITIS ARE DANGEROUS, EARLIER DIAGNOSIS AND EARLIER TREATMENT ARE ABLE TO INHIBIT MMP-2 PROTEIN EXPRESSION,AND SAVE THE PATIENTS IN TIME.