硒对微波辐照致PC12细胞损伤的保护作用

Protective effect of selenium on damaged PC12 cells induced by microwave irradiation

目的 探讨硒对微波致神经细胞损伤的保护作用。方法 以离体培养的PC12细胞为研究对象 ,采用 65mW cm2 的微波照射 2 0min进行致伤 ,以细胞存活率、MDA含量、SOD和GSH Px活性为实验指标反映Na2 SeO3预处理对微波辐照致PC12细胞损伤的影响。结果 微波辐照后PC12细胞存活率明显降低 ,MDA含量增加 ,SOD、GSH Px活性降低 ,而经过Na2 SeO3预处理后再接受微波辐照 ,以上指标的变化明显减轻 ,并且高剂量Na2 SeO3预处理作用更明显。结论 硒可以提高PC12细胞的抗氧化能力 ,保护神经细胞对抗微波辐射导致的细胞损伤。

Objective To observe the damaging effect of microwave on neurons and the protective effect of selenium on damaged neurons after exposure to microwave. Methods In vitro cultured PC12 cells were exposed to 65 mW/cm 2 microwave. The survival rate, SOD activity, GSH-Px activity, and MDA concentration of PC12 cells were detected. The effects of selenium on PC12 cells after exposure to microwave were reflected by these markers. Results Survival rate, activity of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity of PC12 cells decreased, but malondialdehyde (MDA) concentration of PC12 cells increased after microwave radiation. These changes weakened when PC12 cells were exposed to microwave after pretreatment with selenium. This effect was more significant after pretreatment with selenium at the high dose. Conclusion PC12 cells can be damaged by microwave through peroxidation. Selenium pretreatment can promote the antioxidation and protect PC12 cells from damages induced by microwave.