CD_(44)V_6和p16基因蛋白的表达与非小细胞肺癌生物学行为关系的研究

Relationship between expression of CD_(44)V_6,p16 protein and biological behavior of human non-small cell lung cancer

目的 :探讨CD44V6及p16基因蛋白在非小细胞肺癌 (NSCLC)组织中的表达及临床意义。方法 :应用免疫组织化学PictureTM通用型二步法检测 44例NSCLC组织、10例正常支气管黏膜组织、8例肺良性病变中CD44V6和p16的表达情况。结果 :CD44V6在NSCLC中的阳性表达率 68 2 % ,与临床分期、淋巴结转移成正相关 ,与病理类型、组织分化程度无关。而在正常支气管黏膜组织及肺良性病变中无阳性表达。p16在正常支气管黏膜组织及肺良性病变中的阳性表达率分别为 90 0 %和10 0 0 % ,在肺癌组织中的阳性表达率为5 4 5 % ,正常支气管黏膜组织与肺癌之间、肺良性病变与肺癌之间的p16阳性表达率差异均有统计学意义 ,P <0 0 5。p16阳性表达率随NSCLC分期增加呈下降趋势 ,但差异无统计学意义 ,P >0 0 5 ,与淋巴结转移成负相关 ,与病理类型、组织分化程度无关。结论 :CD44V6和p16是 2个独立因子 ,在肺癌的发生、发展、转移过程中发挥调控作用 。

OBJECTIVE:To study the expressions of CD 44V 6,p16 proteins in human non-small cell lung cancer and their relationships with the biological behavior. METHODS:The immunohistochemical method Picture TM was used to detect CD 44V 6,p16 proteins expressions in 44 lung cancer,10 normal bronchial mucosa and 8 innocent disease tissues. RESULTS: 1)The expression of CD 44V 6 was negative in normal bronchial mucosa and in innocent disease tissues, 68.18% in non-small cell lung cancer.There were significances in the groups of clinical stage for CD 44V 6 expression.The expression of p16 was 90.00% in normal bronchial epithelial cell (8/8) in innocent disease tissues, 54.54% (24/44) in non-small cell lung cancer. The significant difference was found between normal bronchial epithelial cell and lung cancer, between innocent disease and lung cancer,P<0.05. The expression of p16 was associated with the clinical stage, but the difference had no significance. The incidences of CD 44V 6 and p16 in patients with lymphaden metastasis were significantly different from those in patients without lymphaden metastasis,P<0.05.CONCLUSIONS:CD 44V 6 and p16 are two independent factors involved in regulating carcino-genesis. The levels of CD 44V 6 and p16 may be sensitive predictors for metastasis and prognosis in NSCLC.Chin J Cancer Prev Treat,2004,11(4):367-369