血管内皮生长因子及其受体在胆囊癌中的表达及与微血管计数的关系

Expressions of vascular endothelial growth factor and its receptor in gallbladder carcinoma and their association with microvessel count

目的 :研究胆囊癌中微血管计数(microvesselcount ,MVC)和血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)及其受体Flk 1(fetalliverkinase 1)的表达及三者对肿瘤血管生成和转移的影响。方法 :应用免疫组化方法检测 49例胆囊癌组织MVC、VEGF和Flk 1。结果 :49例胆囊癌MVC值为( 3 4 5± 11 8)个 /HP ,与Nevin临床分期、淋巴结转移和分化程度密切相关P <0 0 5。VEGF表达的阳性率为 63 3 % ( 3 1/4 9) ,在Nevin临床分期S1～S3和淋巴结未转移者中的表达分别显著低于S4～S5和有淋巴结转移者P <0 0 5 ,且与MVC值呈显著相关 ,t =2 0 61,P <0 0 5。Flk 1表达的阳性率为67 3 % ( 3 3 /4 9) ,与VEGF密切相关 ,P <0 0 5。VEGF及其受体Flk 1共阳性表达者的百分率为 49 0 % ( 2 4/4 9) ,其MVC值显著增高 ,t =2 480 ,P <0 0 5。结论 :VEGF及其受体Flk 1的表达和MVC与胆囊癌的血管生成。

OBJECTIVE:To study the microressel count (MVC),expressions of vascular endothlial growth factor (VEGF) and its receptor Flk-1 in gallbladder carcinoma and their effect on tumor angiogenesis and metastasis. METHODS:The stains of VEGF, Flk-1 and MV were completed by the immunohistochemical method in 49 cases of gallbladder carcinoma.RESULTS:The value of MVC was (34.5±11.8)/HP in 49 cases of gallbladder carcinoma,and it was closely correlated with Nevin staging, lymph node metastasis and tumor differentiation,P<0.05. The positive rate of VEGF was 63.3% (31/49),and its expression was significantly lower in cases of Nevin staging S1-S3 and without metastasis than that of Nevin staging S4-S5 and with metastasis,P<0.05,and was markedly correlated with MVC,t=2.061,P<0.05. The positive rate of Flk-1 was 67.3% (33/49) and it was markedly correlated with VEGF, P<0.05. The percentage of the patients with both positive VEGF and Flk-1 expressions was 49.0% (24/49), and their MVC value was markedly higher than that of the others,P<0.05. CONCLUSION:Expressions of VEGF and its receptor Flk-1,and MVC are closely correlated with angiogenesis, invasion and metastasis of gallbladder carcinoma.