儿童小脑髓母细胞瘤几种标记物的免疫组化表达分析

Expression of several marker in children’s medulloblastoma of cerebellum by immunohistochemical staining technique

目的 :研究儿童髓母细胞瘤的组织发生和Ki67、p5 3、C erbB 2与其发生及预后的关系。方法 :采用SP免疫组化法检测 7例正常小脑、2 2例髓母细胞瘤Ki67、p5 3、C erbB 2、GFAP和NSE的表达。结果 :髓母细胞瘤中GFAP、NSE表达率分别为 45 5 %、5 9 1%。髓母细胞瘤中Ki67、p5 3和C erbB 2的表达率显著高于正常小脑 ,P <0 0 5。未分化组、经典型、肿瘤直径 >5cm组髓母细胞瘤Ki67、p5 3高表达 ,P <0 0 5 ;C erbB 2则与肿瘤坏死和肿瘤大小有关 ,P <0 0 5。结论 :髓母细胞瘤起源于原始神经外胚叶组织 ,具有双向分化潜能。Ki67、p5 3和C erbB

OBJECTIVE:To study the tissue origin and the role of Ki67,p53 and C-erbB-2 in children medulloblastoma oncogenesis,and the retationship with the prognosis.METHODS:SP Immunohistochemical staining technique was used to detect the expressions of Ki67,p53,C-erbB-2,GFAP and NSE in 7 cases of normal cerebellum and 22 cases of medulloblastoma.RESULTS:The positive rates of GFAP and NSE were 45.5% and 59.1% respectively.The expressions of Ki67,p53 and C-erbB-2 in medulloblastoma were much higher than those in normal cerebellum,P<0.05.In the groups which was undifferentiated,classical type and the diameter of tumor was bigger than 5 cm the expressions of Ki67 and p53 were higher than those in the others,P<0.05.The expression of C-erbB-2 was correlated with the necrosis and volume of tumor,P<0.05.CONCLUSIONS:Medulloblastoma originates from primitive neuroecto dermal,possesses the potential of double direction differentiation.The expressions of Ki67,p53 and C-erbB-2 are correlated with the oncogenesis and the prognosis.