TRP-1反义核酸抑制恶性黑素瘤细胞增殖的体外及体内研究

The Inhibition of Antisense TRPl in the Proliferation of Malignant Melanoma Cells in vivo and in vitro

目的:观察TRP-1反义核酸在体内、外对恶性黑素瘤细胞增殖的抑制作用,探讨黑素瘤治疗的新途径。方法:构 建TRP-1反义核酸真核表达载体,将其转染恶性黑素瘤细胞,以测定的MTT结果绘制细胞生长曲线;进行黑素瘤细胞体外克 隆形成实验观察,计算各组细胞的克隆形成率;以TRP-1反义核酸转染的黑素瘤细胞及对照组细胞注射裸鼠,观察肿瘤大小 及增长速度。结果:从生长曲线可以看出,TRP-1反义核酸转染的黑素瘤细胞增殖速度明显低于对照细胞;体外克隆形成实验 结果显示:TRP-1反义核酸转染的黑素瘤细胞克隆形成率为52％,而对照组为68％(P<0.01);裸鼠成瘤试验表明,转染TRP- 1反义核酸的细胞成瘤性显著低于未转染细胞及转染载体对照细胞(P<0.01)。结论:TRP-1反义核酸在体内、外均能明显抑 制恶性黑素细胞的增殖,在恶性黑素瘤的治疗中有一定的应用前景。

Objective: To study the inhibition of antisense TRP1 on cell growth of malignant melanoma(MM) and explore a new way for therapy of melanoma. Methods: Antisense TRP-1 recombinant vector was constructed and transfected into MM cells. According to the results of MTT, cell growth curves were drawn and then clonogenic assay was performed in vitro. At last, tumorigenesis assay was undertaken in nude mice in vivo. Results: Cell proliferations of TRP-1 transfected MM cells were inhibited compared with the control cells. The results of clonogenic assay displayed the difference of clonogenic percentage between TRP-1 transfected MM cells (52% , P <0.01) and the control cells (68% , P <0.01). Tumorigenesis assay in vivo 30 days after implantation showed that the growth of TRP-1 antisense vector transfected MM cells was significantly suppressed. Conclusion: Antisense TRP-1 could inhibit the proliferation of MM cells both in vivo and in vitro. It could be expected that antisense TRP-1 be used in the therapy of MM in the future.