E-cadherin和EGFR表达与胃癌浸润转移的关系

Correlation of E-cadherin and EGFR Expression with Invasion and Metastasis in Stomach Carcinoma

目的探讨Ecadherin(Ecad)和EGFR表达与胃癌浸润转移的相关性。方法应用SP免疫组织化学及原位杂交技术,检测50例胃癌组织中Ecad和EGFR表达。结果50例胃癌中Ecad和EGFR蛋白表达阳性率分别为32.0%(16/50)和76.0%(38/50);EcadmRNA和EGFRmRNA表达阳性率分别为38.0%(19/50)和86.0%(43/50),胃癌组织中Ecad表达率显著低于正常胃黏膜上皮和不典型增生组织;EGFR表达率显著高于正常胃黏膜上皮和不典型增生。Ecad低表达及EGFR高表达与胃癌组织分化、TNM分期、淋巴结转移及患者生存期显著相关(P<0.01),与患者性别、肿瘤大小无关(P>0.05),胃癌中Ecad表达与EGFR表达呈负相关(P<0.05)。结论Ecad和EGFR表达与胃癌浸润转移密切相关,检测Ecad和EGFR蛋白及mRNA表达可作为判断胃癌预后的重要指标。

Objective To study the relation between the expression of E-cadherin(E-cad),EGFR and the metastasis,the invasion of stomach carcinoma.Methods E-cad and EGFR were detected by immunohistochemical staining and in situ hybridization in 50 cases of stomach cancer tissue.Results The positive expression rates of E-cad and EGFR were 32.0%(16/50) and 76.0%(38/50) respectively.The positive expression rates of E-cadmRNA and EGFR mRNA were 38.0%(19/50) and 86.0%(43/50) respectively.The expression of E-cad was significantly lower in stomach carcinoam than in normal stomach mucosa and in atypical hyperplasia mucosa,while the expression of EGFR was significantly higher than that in normal stomach mucosa and in atypical hyperplasia mucosa.Low expression of E-cad and high expression of EGFR were closely associated with stomach carcinoma differentiation,TNM stages,lymphatic nodes metastases and survival time(P<0.01),but had no relation to the gender and the size of tumour(P>0.05).The expression of E-cad and EGFR had a negative relation in stomach cancer(P<0.05).Conclusion The expression of E-cad and EGFR were closely associated with metastasis and invasion of the stomach carcinoma.Detection of the expression of E-cad,EGFR protein and the mRNA in stomach cancer might served as important prognostic predictors.