糖皮质激素对SLE患者Th1/Th2类细胞因子mRNA表达及产生的影响

Effects of Glucocorticoids on Th1/Th2 Cytokine Expression in Peripher al Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus

目的探讨糖皮质激素治疗对系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)中Th1/Th2类细胞因子mRNA表达及产生的影响。方法采用逆转录-聚合酶链反应和酶联免疫吸附试验检测糖皮质激素治疗前后SLE患者PBMC中Th1类细胞因子干扰素γ(IFN-γ)和Th2类细胞因子白介素4(IL-4)、白介素10(IL-10)mRNA表达和产生的变化。结果经糖皮质激素治疗后SLE患者PBMC产生IFN-γ,IL-4和IL-10水平分别为(74.08±15.85)pg/mL,(246.43±45.25)pg/mL,(549.52±67.00)pg/mL;mRNA平均表达水平分别为0.3298±0.2181,0.4951±0.2938,0.8671±0.6620。治疗前IFN-γ,IL-4和IL-10水平分别为(113.5±27.6)pg/mL,(272.97±33.78)pg/mL,(625.32±104.61)pg/mL;mRNA平均表达水平分别为1.4094±0.6317,0.6872±0.4713,1.1758±0.6255;治疗后各细胞因子水平均较治疗前显著降低(P<0.01);但IFN-γ较IL-4,IL-10降低更为显著。结论糖皮质激素可通过影响SLE患者细胞因子mRNA表达和产生,调节Th1/Th2类细胞因子网络平衡,可能为糖皮质激素治疗SLE的机制之一。

Objective To investigate the effects of glucocorticoids o n the expression of Th1/Th2 cytokines at mRNA and protein levels in peripheral b lood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SL E). Methods Th1 cytokine (IFN-IL-10) in PBMCs fro m 48 patients with SLE were detected before and after treatment with glucocortic oids by RT-PCR for mRNA expression and ELISA for protein production. The systemi c lupus erythematosus activity measure (SLAM) scores of patients were compared b efore and after treatment. Results After treatment, the expression and product ion of IFN- versus 1.4094 pg/mL; P < 0.01). The reduction of the ex pression of IL-4 and IL-10 was less than that of IFN-At the same time, the SLAM scores after treatment were significantly lower than those before treatment (4.48 Conclusion These res ults support the hypothesis that glucocorticoids may induce a shift from Th1 to Th2 profile of cytokine secretion which might be one of the mechanisms of glucoc orticoid in the treatment of SLE.