去壁酶与酶解方式对曲霉原生质体释放的影响

Effect of Cell Wall-Decomposing Enzymes and Enzymolytic Pattern on Aspergillus Mycelia Protoplast Release

研究了纤维素酶、蜗牛酶、溶菌酶以及菌丝体培养方式和酶解方式对黑曲霉和米曲霉菌丝释放原生质体的效应。发现黑曲霉菌丝原生质体制备最佳条件为固体透析培养菌丝体,2%纤维素酶,在平皿中,28℃和80r/min条件下酶解3h;米曲霉原生质体制备最佳条件为2%纤维素酶+1%蜗牛酶+5mmol/L二硫苏糖醇,酶解时间6h,其它条件与黑曲霉的相同。

Effects of cellulase, snail enzyme, and lysozyme, including their concentration, reaction time, culture pattern and enzymolytic pattern on mycelia protoplast release of A.niger and A.oryzae were studied. It was found that the optimum conditions for preparing A.niger protoplast is solid dialysis culturing its mycelia on Petri dish with 2% cellulase, and shaking at 80 rpm at 28 ℃ for 2 hours; and the optimum conditions for preparing A.oryzae protoplast is 2% cellulase + 1% snail enzyme + 5 m mol/L dithiothreitol react for 6 hours in the same other conditions as that of A.niger .;