α-转化生长因子及乙型肝炎病毒S抗原在人肝细胞肝癌及肝硬变中的表达及意义

EXPRESSION AND SIGNIFICANCE OF TRANSFORMING GROWTH FACTOR-α AND HEPATITIS B SURFACE ANTIGEN IN HUMAN HEPATOCELLULAR CARCINOMA AND LIVER CIRRHOSIS TISSUES

目的 探讨 α-转化生长因子 (TGF- α)及乙型肝炎病毒 S抗原 (HBs Ag)在人原发性肝细胞肝癌 (HCC)及肝硬变(L C)组织中蛋白和分子水平上的表达及意义。方法 运用免疫组织化学和原位杂交方法对 70例 HCC及 38例 L C组织进行研究 ,并采用 5例正常人类肝脏作为对照。结果 TGF- α在 HCC及其癌旁组织、L C组织中的阳性率分别为 74 .3% (5 2 / 70 )、88.1% (5 2 / 5 9)和 84 .2 % (32 / 38) ,TGF- α在高分化 HCC中 TGF- α的阳性率明显高于中、低分化的 HCC(P<0 .0 5 ) ,HBs Ag在 HCC及其癌组织、L C组织中的阳性率分别为 2 1.4 % (15 / 70 )、79.7% (47/ 5 9)、73.7% (2 8/ 38) ,二者的表达在 HCC癌旁组织 (γ癌旁 =0 .34)及 L C组织中呈显著正相关 (γL C=0 .36 ) ;在再生活跃或不典型增生的肝细胞中 ,可见 TGF- α和 /或 HBs Ag阳性 ;在 HCC及其癌旁组织中 ,TGF- α的原位杂交阳性率略高于免疫组织化学效果。结论 HBV感染与 HCC的发生有关 ,过量表达的 TGF- α可能参与 HBs Ag所致的损伤肝细胞的再生过程 ,持续的 TGF- α表达可引起肝细胞的增殖 ,从而导致肝细胞的不典型增生 ,最后致使 HCC的发生。

Objective To evaluate the expression and significance of transforming growth factor-alpha(TGF-α)and hepatitis B surface antigen(HBsAg)in human hepatocellular carcinoma(HCC) and liver cirrhosis(LC) tissues.Methods Seventy specimens of HCC and thirty-eight specimens of LC were detected by immunohistochemistry and in situ hybridization.Five specimens of normal human liver were used as control tissues.Result The expression rates of TGF-α in HCC and its peripheral tissues,LC tissues were 74.3%(52/70),88.1%(52/59) and 84.2%(32/38).TGF-α expressing positive rate in well differentiated HCC was usually higher than that in moderately and poorly differentiated HCC(P<0.05).The expression rates of HBsAg in HCC and its peripheral tissues,LC tissues were 21.4%(15/70),79.7%(47/59)and 73.7%(28/38).There was prominently positive correlation between TGF-α and HBsAg in HCC peripheral tissues(P<0.05,γ=0.34) and LC tissues(P<0.05,γ=0.36).TGF-α was closely correlated with HBsAg in regenerating and/or dysplasia of liver cells.In situ hybridization showed the level of TGF-α mRNA was appreciably higher than that of immunohistochemistry.Conclusion HBC infection was closed correlated with hepatocarcinogenesis.The overexpression of TGF-α in the liver seemed to be associated with the regeneration of hepatocytes injured by HBsAg .The continued expression of TGF-α might lead to dysplasia of liver cells.Also,it might be related closely to the development of HCC.