TGF-β1、CycinE、P27在原发性胆囊癌中表达及临床意义

THE EXPRESSION AND CLINICAL SIGNIFICANCE OF TGF-β1、CycinE、P27 in GALLBLADDER CARCINOMA

目的 检测 TGF- β1、Cycin E、P2 7在原发性胆囊癌中表达 ,研究它们的蛋白产物在原发性胆囊癌发生、发展过程中表达特点及相互关系。方法 应用免疫组化 S- P法检测 36例原发性胆囊癌中 TGF- β1、Cycin E、P2 7的表达 ,并以同期 2 0例慢性胆囊炎作对照。结果 1.TGF- β1、Cycin E基因蛋白产物阳性表达率显著高于其它相应良性对照组。P2 7蛋白表达率显著低于相应良性对照组。 2 .TGF- β1、Cycin E蛋白在有淋巴结或远处转移、Nevin - 期患者中明显增高于无转移、Nevin - 期的患者。P2 7在高中分化、无淋巴结或远处转移、Nevin - 期的患者中阳性表达率明显高于低分化、有转移、Nevin - 期患者。 3.TGF- β1与 Cyclin E、P2 7两者分别呈正、负相关。 4 .TGF- β1、Cycin E阳性患者生存率明显低于 TGF- β1、Cycin E阴性患者 ,P2 7阳性患者生存率高于 P2 7阴性患者。L ong- rank检验显示生存率差异都有显著性。结论 联合检查 TGF- β1、Cycin E、P2 7在原发性胆囊癌中表达有助于反映原发性胆囊癌生物学特性、为预后判断提供参考指标 ,并为干预性基因治疗提供实验依据。

Objective This study was to investigate the expression of TGF-β1、CycinE、P27 in gallbladder carcinoma and its relation to development of the carcinoma.Methods The expression of TGF-β1、CycinE、P27 in gallbladder carcinoma were detected by S-P immunohistochemical staining,20 cases of chronic cholecystitis were collected as the control group.Result 1.The positive rate TGF-β1 was higher in metastasis or NevinⅣ-Ⅴ stage group of gallbladder carcinoma than in non-metastasis or NevinⅠ-Ⅲ stage group(P<0.05).In metastasis or low differentiation there were also higher expression of CyclinE(P<0.05).The positive rate P27 was inversely proportional to the middle correlated with the expression of TGF-β1,P<0.05.The expression of CyclinE was inversely correlated with the expression of TGF-β1,P<0.05.4.There was significant difference in survival time bwtween patients with TGF-β1+and TGF-β1-or P27+and P27-or CyclinE+and CyclinE-.There were statisically correlations between the first group and the second group.Conclusion The detection of TGF-β1、CycinE、P27 may be useful in assessing the development of the cancer,judging prognosis and eventually render a possible target for novel therapeutic strategies.