瓦斯爆炸伤大鼠脑组织蛋白激酶Cαm RNA与c-fos基因表达的关系

A study on relationship between rat brain tissues hurts of the gas explosion and the expression of Protein kinase CamRNA and c-fos gene

目的探讨蛋白激酶CαmRNA(PKCαmRNA)与c-fos基因蛋白(c-fos)的表达在瓦斯爆炸致大鼠脑损伤时的变化规律。方法建立瓦斯爆炸伤大鼠实验模型。采用原位杂交技术测定大鼠脑组织蛋白激酶CαmRNA,采用免疫组化染色法测定c-fos基因蛋白。结果PKCαmRNA与c-fos对照组大鼠脑组织均有少许表达。大脑皮质、海马PKCαmRNA24h、48h及72h组的表达明显增强,且48h达高峰(P<0.01);脑皮质、海马c-fos基因蛋白0.5h表达开始有明显增加,4h达高峰,以后强度逐渐降低,第5日基本恢复正常。结论瓦斯爆炸伤致脑组织缺血缺氧可促进脑神经细胞蛋白激酶CαmRNA的表达,PKCαmRNA可调节c-fos基因的表达,二者均参与了神经细胞的损伤过程。

Objectives To explore relationship between rat btain tissues hurts of gas explosion and the expresion of Protein Kinase CαmRNA. Methods Build up rat hurt model of gas explosion.In Situ Hybridization(IDH)technique was used to test Protein Kinase CαmRNA.Immunohistochemical Assays(IHA)was used to determine c-fos gene protein. Results Only a little a mount expression of PKCαmRNA and c-fos of the control group was detected.The expression of the cerebral cortex and hipocampus of PKCαmRNA 24 h,48 h and the 48 h increased obviously,and the 48 h reached the peak of expression;(t=4.12 P<0.01).The expression of c-fos protein of the cerebral cortex and hippocampus started to increase obviously at 0.5 h and the 4 h reached the peak,then the strength lowered gradually and the expression level came back normal level on fifth day. Conclusion The anoxia of brain tissues due to the gas explosion may promote the expression of PKCamRNA,and PKCamRNA could regulate the expression of the gene of c-fos.Both PKCamRNA and the gene of c-fos are involved in harmful processes to the nerve cells.