纳米级四氧化三铁的药物动力学和组织分布研究

Pharmacokinetics and tissue distribution of nanometer Fe3O4

目的 研究纳米级四氧化三铁在家兔体内的药物动力学和在小鼠体内的分布与排泄。方法 将纳米级四氧化三铁静脉注射到家兔体内, 用原子吸收光谱法测量血药浓度, 将血药浓度 时间数据用3P87程序拟合, 计算出药物动力学参数。另将纳米级四氧化三铁注入小鼠体内, 测量小鼠不同脏器中的铁含量。收集给药后 48 h内的尿液和粪便, 测量其中的铁含量。结果 家兔静脉注射四氧化三铁后呈双室模型代谢。四氧化三铁在小鼠体内主要分布在肝脏和脾脏等网状内皮细胞和吞噬细胞较多的脏器, 并且在小鼠体内排泄缓慢。结论 四氧化三铁在家兔体内呈双室模型代谢, 在体内的分布依次为: 肝脏、脾脏、心脏和肾脏。

OBJECTIVE To determine the pharmacokinetics of nanometer monodisperse ferromagnetic nanoparticles iron oxid in rabbits’body and its distribution and excretion in rats METHODS Nanometer Fe 3O 4 was injected into rabbits Plasma concentration of nanometer Fe 3O 4 was measured by atomic absorption spectrophotometry The concentration time data of nanometer Fe 3O 4 were fit by 3P87 program automatically and then we calculated the pharmacokinetical parameter We also injected the nanometer Fe 3O 4 into the rats and measured the ferric content in varies splanchna,collected the urine and dejecta within 48 hours and measured the ferric content after injecting the nanometer Fe 3O 4 into rats RESULTS Nanometer Fe 3O 4 was fit to a two compartment open model in rabbits Nanometer Fe 3O 4 was mainly distributed in the liver and the spleen in rats,and its excretion was slow CONCLUSIONS Nanometer Fe 3O 4 was fit to a two compartment open model in rabbits.The nanometer Fe 3O 4 was distributed in the liver,spleen,heart and kidney decreasingly