鱼腥草注射液对膜性肾病大鼠的初步实验研究

Experiment Study on the Effect of Herba Houttuyniae on Membranous Nephritis Rats

目的:探讨鱼腥草注射液对膜性肾病大鼠的影响及作用机制。方法:将造模大鼠分为4组:即模型对照组、鱼腥草注射液20mL/Kg组、鱼腥草注射液10mL/Kg组、地塞米松磷酸钠注射液0.1mg/Kg组,连同原有正常对照组,共5个组。实验结束时测定24h尿蛋白定量,并作光镜下观察及免疫组化染色。结果:鱼腥草注射液大、小剂量组24h尿蛋白定量较模型对照组显著减少(P<0.05);光镜下鱼腥草大剂量组较模型组肾小球周围炎性反应明显减轻,血管内仅有少量红细胞,体视学统计鱼腥草大小剂量组分叶肾小球较模型组有显著性减少(P<0.05),TGF-β1免疫组化染色结果鱼腥草大剂量组肾小球及周围组织着色明显较浅,免疫反应明显减轻。结论:鱼腥草注射液能降低造模大鼠的24h尿蛋白定量,对造模大鼠有减轻其病理损伤的作用。

Objective:The study was designed to observe the effect of herba houttuyniae on SD MN rats and discuss it's mechanism of action.Methods:SD rats were made to membranous nephritis model by cation bovine serum albumin and divided into4groups,the model group,the high-dose and low-dose houttuynia group,Dex group.Heathy rats were used as normal contral group.Twenty-four hours' urinary protein content and plasma albumin,blood lipid were tested by the end of experiment.Pathological changes were observed by using light and used immunohistochemistry staining test.Resutts:As compared to model group,high-dose and low-dose houttuynia group rats showed less urinary protein excretion(P<0.05),Light、immunohistochemistry staining all showed that the pathologic changes in the high-dose and low-dose houttuynia group were lesser than that of the model group.Especially the high-dose houttuynia group.Conclusion:Houttuyniae can reduce urinary protein content.Houttuyniae also can reduce the pathologic changes of SD rats were made to membranous nephritis model by cation bovine serum albumin.