载药微乳液相行为的研究

Studies on the Phase Behaviors of Drug-loading Microemulsions

提出了一种基于相图研究实现纳米药物载体可控制备的方法，采用微乳液控温相图绘制装置绘制了硬脂酸聚烃氧(40)酯(S-40)/聚氧乙烯聚氧丙烯醚嵌段共聚物(F-68)/单硬脂酸甘油酯(GMS)/水体系的拟三元相图，基于电导率测定值确定了微乳液的结构(W/O、双连续相和 O/W)，该体系同时存在液晶区域，乳化剂S-40/F-68的质量比为7∶3，研究了脂溶性药物维甲酸(RA)对微乳液相行为的影响，结果表明 RA 的加入对微乳液的相行为影响较小，基于相图研究结果制备了维甲酸固体脂质纳米粒(RA-SLN)，亚微米粒度分析仪(PCS)测定的平均粒径和透射电镜测试都表明 RA-SLN 为10nm 左右的球状粒子。

A new method was presented for controllable preparation of nanoscale drug carriers based on the phase behaviors of microemulsions. The pseudoternary phase diagrams for the system polyoxyethylene (40) stearate (S-40)/polyoxyethylene-polyoxypropylene(F-68)/Glycerol monostearate(GMS)/water were obtained at 60 degreesC using home-made apparatus with temperature control. Different structures of microemulsion(W/O, B. C. and O/ W) were distinguished by the conductivity measurement, and liquid crystal region was found to be in existence. The mass ratio of S-40 and F-68 was selected to be 7:3. It was concluded that the introduce of retinoic acid (RA) has little influence on the phase behavior of drug-loading microemulsions. Solid lipid nanoparticle (SLN) of retinoic acid was prepared based on the phase behavior results. The mean particle size of RA-SLN determined by PCs is about 10 nm, and TEM images indicate that particles of RA-SLN are spherical with a size about 10 nm. The study on the phase behaviors of drug-loading microemulsions has been proved to be very significative for the controllable preparation of SLN.