胃癌细胞uPA表达与腹膜种植潜能的相关性研究

uPA expression of gastric cancer cell lines and its correlation with peritoneal seeding

目的 探讨不同胃癌细胞系尿激酶型血浆纤溶酶原激活因子 (uPA)的表达及其与腹膜种植能力的关系。方法 采用半定量RT PCR、Westernblot和ELISA方法 ,比较不同胃癌细胞系 (AGS、SGC790 1、MKN4 5和MKN2 8)uPA表达和uPA活性的差异。将胃癌细胞直接注入裸鼠腹腔 ,建立腹膜种植模型 ,通过观察裸鼠生存时间等方法 ,比较不同胃癌细胞系的腹膜种植潜能。结果 uPA表达以SGC790 1细胞最高 ,uPA活性以MKN4 5细胞最高 ,AGS细胞uPA表达和活性均最低。AGS未发生腹膜种植肿瘤 ;SGC790 1和MKN4 5均出现大小不等的腹膜种植肿瘤 ;MKN2 8出现伴有血性腹水且大小相似的腹膜种植肿瘤 ;但MKN4 5最早形成腹膜种植肿瘤 ,并且该组裸鼠的生存时间最短。结论 各株胃癌细胞的uPA表达在转录和翻译水平相一致 ,但uPA的表达量和uPA活性大小有明显差异 ,并且这二者与腹膜种植能力呈正相关。

Objective To study the correlation between expression of urokinase type plasminogen activator (uPA) and capability of tumor cell seeding to the peritoneal membrane by different gastric cancer lines. Methods Expression of uPA in 4 human gastric cancer cell lines was examined by semi quantitative RT PCR, ELISA and Western blot. uPA activity was determined by an assay kit. After ip inoculation of cancer cells to nude mice, tumors on peritoneal membrane was grossly examined for tumor cell seedings. Results SGC7901 was the highest in uPA expression among human gastric cancer cell lines AGS, SGC7901, MKN45, and MKN28. MKN45 had the strongest uPA activity, while AGS was lowest in both uPA expression and activity. Peritoneal seeding tumors of various sizes were observed in mice inoculated with SGC7901 and MKN45 cells. In addition to peritoneal seedings, bloody ascites was present in mice inoculated with MKN28. The MKN45 inoculated mice took the least time to develop tumors and had the shortest surviving period. No peritoneal seeding was seen in mice inoculated with AGS cells. Conclusion Three of 4 human gastric cancer cell lines studied express uPA mRNA and activity, which correlate with their peritoneal seeding potentials.