肝细胞生长因子及其受体在非小细胞肺癌中的表达及意义

Expression of hepatocyte growth factor and its receptor in non -small cell lung carcinoma and its significance

目的 检测肝细胞生长因子 (HGF)及其受体 (c- Met)在正常支气管黏膜上皮和非小细胞肺癌(NSCL C)中的表达 ,分析其与 NSCL C临床病理参数的关系及 HGF、c- Met相互作用方式。方法 应用免疫组化S- P法对 14例正常支气管黏膜上皮组织和 4 5例 NSCL C组织中的 HGF、c- Met进行检测。结果 14例正常支气管黏膜上皮组织 HGF、c- Met表达率分别为 14 .3% (2 / 14 )和 2 1.4 % (3/ 14 ) ,而在 4 5例 NSCL C组织中分别为 5 1.1%(2 3/ 4 5 )和 6 0 .0 % (2 7/ 4 5 )。 HGF及其受体在肿瘤和正常组织中的表达 ,差异有显著性 (P=0 .0 15 ,P=0 .0 12 )。HGF的表达与淋巴结转移相关 (P=0 .0 2 6 )。c- Met的表达与组织类型、分化程度相关 (P=0 .0 14 ,P=0 .0 2 5 )。HGF的表达与 c- Met的表达呈明显正相关 (P=0 .0 0 2 ,r=0 .4 72 ) ,两者均阳性者为 19例 (42 .2 % ) ,其中 13例 (6 8.4 % )表现为自分泌形式 ,6例 (31.6 % )表现为旁分泌形式。结论 HGF及 c- Met主要通过自分泌作用参与 NSCL C发生、发展和演进 ,两者在腺癌中均呈高表达 ,可作为肺腺癌诊断和预后判断的指标。

Objective To detect the expression of HGF and c-Met proteins in normal bronchial epithelial(NBE) and non-small cell lun g carcinoma(NSCLC), to explore the expression relationship with clinic pathlogic parameters of NSCLC, and to investigate function between HGF and c-Met. Methods Immunnohistochemistry(S-P method) was adopted to detect the HGF and c -Met proteins expression in 14 cases of NBE and 45 NSCLC. Results In NBE positive rates of HGF and c-Met were 14.3% (2/14) and 21.4%(3/14), r espectively, but in all of NSCLC were 51.1%(23/45) and 60.0%(27/45), respectivel y. The difference of HGF and c-Met expression was significant between NSCLC and NBE (P=0.015,P=0.012). HGF expression was correlated with lymph node me tastasis (P=0.026). c-Met expression was related to differentiation degree and histotype (P=0.014,P=0.025). There was positive relationship betwe en the express ions of HGF and c-Met (P=0.002, r=0.472) in NSCLC. Ninteen cases of 45 NSCLC (42.2%) were found to co-express HGF and c-Met, 13 of 19(68.4%) were aut ocrine, 6 of 19(31.6%) were paracrine. Conclusions HGF and c-M et is involved in formation and progression of NSCLC mainly by autocrine. Both h ave high level expression in adenocarcinoma and might be diagnostic and prognost ic factors of pulmonary adenocarcinoma.