骨髓基质干细胞与骨基质明胶复合培养体内异位成骨的实验研究

Study on ectopia osteogenesis of the composite of bone mesenchymal stem cells and bone matrix gelatin in vivo

目的:探讨骨髓基质干细胞(MSC)与骨基质明胶复合培养体内异位成骨的可行性。方法:将SD大鼠来源的MSC与同种异体的骨基质明胶复合培养后植入SD大鼠背部竖脊肌肌膜内,分别于术后不同的时间点处死大鼠,标本进行碱性磷酸酶(ALP)活性测定及组织形态学观察。结果:实验组标本自术后第3周ALP活性起就表现出阳性,第4周MSC与骨基质明胶复合体内大量成骨。结论:MSC与骨基质明胶复合培养体内异位成骨完全可行的。

Objective: To study the feasibility of ectopia osteogenesis of the composite of bone mesenchymal stem cells and bone matrix gelatin in vivo. Methods: Ten SD rats were used in the study. After each SD rat was anesthetized, its dorsal skin was cut up and then its dorsal muscles were exposed. The composite of labeled rMSCs and bone matrix gelatin which had been culturing for 72 hours was implanted into the right side dorsal muscles. Simultaneously, the bone matrix gelatins were implanted into the left side dorsal muscles. After 1、2、3、4、8 weeks, every two rats of ten were killed, and then the implantations were obtained. The bone formations were observed by morphological examination, histochemistry and immunohistochemistry. Results: Bone formations could be found in all the implantations, but the quantity of the bone formations of experimental groups (implanting the composite of rMSCs and BMG) was more than that of control groups (implanting BMG alone). Tumorous cell was not found in all of the implantations. Conclusions: The ectopia osteogenesis of the composite of bone mesenchymal stem cells and bone matrix gelatin is feasible in vivo. The quantity of bone formations of implanting the composite of rMSCs and BMG is more than that of implanting BMG alone.