表皮生长因子受体Ⅲ型突变体在肝细胞癌中的表达及意义

Expression and Significance of Epidermal Growth Factor Receptor Variant Type Ⅲ in Hepatocellular Carcinoma

背景与目的有许多证据表明表皮生长因子受体Ⅲ型突变体(epidermal growth factor receptor variant typeⅢ,EGFRvⅢ)在一系列恶性肿瘤的发生发展中起重要作用,但EGFRvⅢ与肝癌的关系目前尚未明确。本研究旨在探讨EGFRvⅢ在人肝细胞癌(hepatocellularcarcinoma,HCC)癌组织中的表达,并分析其意义。方法用免疫组化SP方法检测55例HCC癌组织及癌旁肝组织中EGFRvⅢ的表达。结果HCC癌组织中EGFRvⅢ的阳性率(61.8%,34/55)高于癌旁肝组织中的阳性率(38.2%,21/55),两者间有显著性差异(P<0.05)。人肝癌组织中EGFRvⅢ蛋白的检出率与临床分期、门静脉癌栓、肝外转移、术后复发、肿瘤直径大小呈显著性相关,而与肿瘤数目、分化程度、血清AFP水平或HBsAg状况、癌旁肝硬化无显著性相关。结论EGFRvⅢ在肝癌组织中有较高表达,且与肝癌的发展及术后复发等有关。

BACKGROUND & OBJECTIVE: Many evidences show that epidermal growth factor receptor variant type Ⅲ (EGFRvⅢ) plays an important role in initiation and progression of various cancers. But the relationship between EGFRvⅢ and hepatocellular carcinoma (HCC) is unclear. This study was to explore the expression and significance of EGFRvⅢ in human HCC tissues. METHODS: Immunohistochemical staining (IHC) was used to detect the expression of EGFRvⅢ in 55 specimens of HCC tissues and their adjacent non-cancerous liver tissues. RESULTS: Positive rate of EGFRvⅢ in HCC tissue was significantly higher than that in adjacent non-cancerous liver tissue [61.8% (34/55) vs. 38.2% (21/55), P < 0.05]. Positive rate of EGFRvⅢ in HCC tissue was significantly correlated with clinical stage,portal vein tumor thrombus,presence of extrahepatic metastasis, tumor recurrence, and the diameter of tumor, but not correlated with the number of foci, serum alpha-fetoprotein (AFP) level, serum HBsAg status, tumor differentiation, and cirrhosis in adjacent liver tissue. CONCLUSION: EGFRvⅢ is over-expressed in human HCC tissues, and relates to progression and recurrence of HCC.