厌氧除磷种源的筛选与厌氧除磷条件的研究

Screening for the phosphate reducer and study on their dephosphorization conditions

通过分批培养,考察各接种物培养始末总磷浓度的变化,比较了牛粪、猪粪、鸡粪以及来自两个不同污水处理厂的厌氧污泥的除磷能力,试验证明猪粪的除磷效果最佳.在此基础上,将猪粪与两种厌氧污泥按质量比3∶1∶1混合作为接种物,研究了厌氧生物除磷的条件,结果表明葡萄糖和蛋白胨分别是厌氧生物除磷的良好碳源和氮源;适宜的初始pH6 5,适宜的培养温度35℃.在适宜的除磷条件下,培养至第4天,猪粪与厌氧污泥混合物对总磷的去除率达到最大值(21 31%).外加钼和其它微量元素对厌氧生物除磷没有效应,表明试验所用的培养液中,不缺少这些元素.另外,外加还原剂硫化物对厌氧生物除磷也没有明显的作用,表明培养液中的有机物质所致的低氧化还原电位已能满足厌氧生物除磷的要求.

The biological reduction for phosphate to phosphine can be used to develop a new biotechnology for phosphate removal from wastewater. A suitable inoculum should be prepared and the conditions of the reaction should be clear. By measuring the contents of total P both at the beginning and at the end of batch cultivation, the phosphate reduction activities of cow manure, swine manure, chicken manure and two kinds of anaerobic activated sludge from different WWTP were compared, and the swine manure was found to be the best of the inocula. Based on that, the swine manure was mixed with two kinds of anaerobic activated sludge at the mass ratio of 3∶1∶1, and the mixture was then used as the seeding sludge to test the optimal conditions for phosphorus removal in anaerobic surroudings. The results showed that the optimal carbon and nitrogen sources were glucose and peptone, respectively. The optimal initial pH value was 6.5 and the temperature was 35℃. Under the optimal conditions the maximal phosphate removal efficiency was 21.31% during the four-day cultivation. No obvious effect on the anaerobic phosphorus removal was found when molybdenum and other trace elements as well as sulfide as a reducing agent were added into the culture media.