福清黑松醇溶蛋白的群体遗传分析

Population Genetics Diversity of Gliadin of Pinus thunbergii from Fuqing

采用聚丙烯酰胺凝胶电泳技术,对福清黑松群体进行醇溶蛋白的多样性分析,共获得40种图谱,9个等位基因位点。对这些位点的统计分析表明,福清黑松的多肽位点百分率P=55.56%, 等位基因平均数A=3.00,平均等位基因有效数目Ae=2.28,预期杂合度He=0.533,平均实际杂合度Ho=0.402,固定指数F仅为0.246>0。该群体表现出遗传多样性水平较高,但该群体偏离了Hardy-Weinberg平衡定律,原因可能是取样偏差、群体中个体密度分布不均和个体生长状况不好,不能产生充分的随机交配,出现遗传漂移导致的。

Genetic variation of gliadin from endosperm were examined of 40 individuals of Pinus thunbergiipopulation in an area of 7.1 hm2 Fuqing natural forest in Fujian Province. Forty patterns of gliadin diversity and 9 loci were obtained by using polycrylamide gel electrophoresis. The results indicate that the proportion ofpolymorphic loci (P) was 55.56, mean number of alleles per locus (A) was 3.00, mean effective number of alleles per locus (Ae) was 2.28, mean expected proportion of heterozygous loci (He) was 0.533, mean observedproportion of heterozygous loci (Ho) was 0.402, and the fixation index (F) 0.246>0. The population exhibits high genetic diversity, which deviates from Hardy-Weinberg's equilibrium law. This is probably due to sampling error, or unevenness of stand or poor growth of the individuals, resulting in inefficiency of radom mating and genetic drift.