核糖核酸酶抑制因子对H_2O_2所致的细胞过氧化损伤的保护作用

Protection Effects of Ribonuclease Inhibitor on the Cells Peroxidative Damage Injured by H_2O_2

背景与目的:核糖核酸酶抑制因子(ribonuclease inhibitor,RI)是广泛存在于哺乳动物细胞质中的一种酸性糖蛋白,根据RI分子组成富含巯基的特点,研究其对过氧化氢(H2O2)损伤的小鼠黑色素瘤B16细胞的影响。 材料与方法:用不同浓度的H2O2分别作用于B16细胞及转染有RI cDNA并且RI过表达的B16细胞,对比损伤前后两者的细胞存活率、LDH漏出量和细胞内MDA含量差别、以及细胞内抗氧化酶类GSHPx、SOD、cAT、XOD活性的差别。结果:与B16细胞相比,RI过表达的B16细胞在H2O2作用下存活率高,LDH漏出量、MDA含量明显减少,细胞内GSHPx、SOD、CAT活性下降幅度减小。结论:RI具有抗氧化功能,能够减轻H2O2所致的细胞过氧化损伤。

BACKGROUND & AIM: Ribonuclease inhibitor (RI) is an acidic cytosolic glycoprotein. It has plenty of reduced thiol groups. According to this point, we study the effects of RI on the melanoma B16 cells injured by H2O2. MATERAIL AND METHODS: The transfected B16 cells with RI cDNA in which RI was overexpressed and the control B16 cells were treated with different dose of H2O2 respectively. The different changes of cell viability, LDH leakage, MDA contents and the activities of antioxidative enzymes were investigated by comparison between these two kinds of cells. RESULTS: The transfected B16 cells had higher viability, less LDH leakage and MDA contents, and higher activities of GSHPx,SOD and CAT. CONCLUSION: This result showed that RI had antioxidant activity and it was able to protect cells from peroxidative injuries.