三羟异黄酮对BALB/c-3T3细胞的转化作用

Study on the Effects of Genistein on the Transformation of BALB/c - 3T3 Cells

背景与目的:研究三羟异黄酮(GEN,Genistein)在体外细胞转化中的作用。材料与方法:用改进的BALB／c-3T3细胞转化方法观察GEN组、B[a]P(10 μmol/L)+GEN组以及MCA(3-甲基胆蒽,0.2 μg／ml)+TPA(豆蔻酸乙酸大戟二萜酯,0.1 μg／ml)+GEN组中细胞转化的情况,同时测定突变型P53蛋白及PTK(酪氨酸蛋白激酶)的活性。每个组别GEN设4个剂量,分别是10、30、90、270 μmol/L。结果:与对照组相比,GEN≥30 μmol/L剂量组细胞转化率升高,GEN(30 μmol/L)+B[a]P(10μmol/L)细胞转化率高于B[a]P组,发生转化的细胞中突变型P53值升高。然而,在MCA+TPA模式下,30μmol/L以上GEN抑制MCA+TPA诱导的细胞转化,PTK值 随GEN升高而下降。结论:在不同模式下GEN的效应不同。GEN有诱导细胞恶性转化的作用,但在一定条件下又可抑制细胞恶性转化。

BACKGROUND & AIM: To study the transforming effects of genistein on BALB/ c-3T3 cells. MATERIAL AND METHODS: We used the improved BALB/c-3T3 cell transformation assay to observe the transformation of cells treated by: GEN, B[a]P(10μmol/L) + GEN and MCA(0.2μg/ml) +TPA(0.1 μg/ml) + GEN,at the same time, to detect mutant P53 and PTK activity .RESULTS: When GEN ≥30 μmol/L ,the transforming frequency of BALB/c-3T3 cells was significantly increased compared with Contnol(DMSO 0.5 %) (P < 0.01) .The transforming frequency induced by B[a]P (10 μmol/L) +GEN(30μmol/L)was much higher than that by B[a]P(10 μmol/L). Expression of mutant P53 was also increased in the group treated by B[a]P(10 μmol/L) + GEN(30μmol/L), B[a]P(10μmol/Dand GEN(30 μmol/L) However,GEN≥30 μmol/L could inhibit the cell transformation induced by MCA + TPA;PTK activity also decreased with increasing the dose of GEN. CONCLUSION: Our results suggested that GEN could induce cell transformation itself,on the other hand,it could also inhibit cell transformation under certain conditions.