中度嗜盐或耐盐放线菌生长对阴离子选择性研究

Study on the Growth for Selectivity to Negative Ion of Moderately Halophilic Actinomycetes

长期以来Cl被作为主要阴离子来分离和培养中度嗜盐放线菌,但是盐湖中还含有NO3、SO42、CO32和HCO3等阴离子。培养基中只加入含有Cl的盐,不能完全模拟出自然界的环境,因此对中度嗜盐或耐盐放线菌的研究会有一定的限制。实验通过研究部分中度嗜盐或耐盐放线菌菌株生长对阴离子的选择影响,结果发现中度嗜盐或耐盐放线菌对部分阴离子如NO3和SO42没有太大的选择性,Cl可以被NO3和SO42所代替;但有些阴离子如CO32和HCO3仅对拟诺卡氏菌属(Nocardiopsis)影响较小,对盐碱环境中的其他属的放线菌,如普氏菌属(Prauserella)、链单孢菌属(Streptomonospora)、糖单孢菌属(Saccharomonospora)等影响较大。

Cl^-was used as main negative ion long time for isolation and culture of moderately halophilic or halotolerant actinomycetes, but there are other negative ions such as NO_3^-、SO_4^(2-)、CO_3^(2-)and HCO_3^-in salt lake. Media with only Cl^-could not reflect their living natural environments, which was limited to study on moderately halophilic or halotolerant actinomycetes. In this study, the results show that some negative ion, such as NO_3^-and SO_4^(2-), have not much more selectivity to moderately halophilic or halotolerant actinomycetes, Cl^-can be substituted by NO_3^-and SO_4^(2-). CO_3^(2-)and HCO_3^-has little effect on the genus Nocardiopsis,but have much more on other genera such as Prauserella,Streptomonospora and Saccharomonospora, etc. These results will be some instructive to the isolation and cultivation of moderately halophilic or halotolerant actinomycetes.