摘要
以降解聚-β羟基丁酸酯(PHB)的青霉(Penicilliumsp.)DS9713a为出发菌株,通过紫外线(UV)诱变分生孢子,采用透明圈初筛和摇瓶复筛,获得酶活高于原始菌株的突变株5株,其中DS9713a-CS01突变株的PHB解聚酶活力高于对照97.42%,并对其酶学性质进行了初步研究。
Conidial suspension was obtained from the Penicillium sp. DS9713a which could decompose PHB. Through screening a lot of mutants with the methods of transparent zones and flask culture, five strains were selected from starting strain treated with UV and their activity were higher than the original .The best mutant strain named DS9713a-CS01, it's enzyme activity was as 197.42% high as that of the original ,furthermore, the characters of crude enzyme were studied.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第1期38-43,共6页
Microbiology China
基金
国家自然科学基金资助项目(No.29774031
29834102)~~
