椒葛软胶囊中葛根素的含量测定

Determination of puerarin in Jiaoge soft capsules

目的:建立椒葛软胶囊中葛根素含量测定的方法。方法:采用高效液相色谱法,色谱柱为Hypersil ODS柱(250mm×4.6mm,2.5μm)。流动相为CH_3OH-CHCl_3-1.5%冰醋酸(22:1:77),波长为250nm。结果:葛根素的线性范围为0.042～0.210mg·mL^(-1),r=0.9999;平均加样回收率为100.4%,RSD为1.17%。结论:用高效液相色谱法测定椒葛软胶囊中的葛根素含量,方法准确、简便易行。

Objective:To establish a HPLC method for determination of puerarin in Jiaoge soft capsule. Methods:The chromatographic condition was as follows:a column of Hypersil ODS (250mm×4.6mm,2.5μm),a mobile phase consisting of CH_3OH-CHCl_3-1.5% glacial acetic acid (22: 1: 77),and a UV detector at 250nm. Results: The method has a linear range of 0.042～0.210mg·mL^(-1), r=0.9999, and a mean recovery rate of 100.4% with a RSD of 1.17%. Conclusion:The HPLC methods are simple and accurate,and can be used in quality control of Jiaoge soft capsules.