内毒素诱导小鼠β-防御素-3(mBD3)基因急性时相表达与肝脏损伤的关系

Relation of the mice β-defensin3 gene acute-phase expression and the liver injury induced by endotoxin

目的 :探讨急性时相反应时 β -防御素基因表达与内毒素性肝损伤的关系。 方法 :小鼠腹腔注射内毒素建立急性时相反应 ,经Northern -blot检测各组织中mBD3mRNA及表达的剂 -效、时 -效关系。结果 :仅在肝脏组织中检测到mBD3mRNA。内毒素诱导mBD3mRNA在肝脏中的表达存在最小诱导剂量。以最小诱导剂量 (1.5 μg/g)注射后 6h才出现mBD3mRNA的表达 ,8～ 10h达峰值 ,12h后表达水平下降。不同剂量内毒素注射后不同时间引起肝脏组织出现不同程度的炎性反应。结论 :mBD3基因在肝脏中的急性时相表达与内毒素引起的肝脏组织炎性反应有关。

Objective:To study the relation of the β-defensin gene expression in acute-phase reactants and the liver lesion after injection of endotoxin.Methods: Murine systemic acute-phase responsive model was established by intraperitoneal injection of LPS .The expression of the mice β-defensin3 mRNA in different tissues and the time- and dose- dependent expression caused by LPS were detected by Northern-blot. Results:The expression of mBD3 gene was only detected in mouse liver after LPS injection .The minimum dose of LPS inducing mBD3 expression in liver was 1.5 μg/g.It was found that mBD3 mRNA was not expressed until 6 hours later and reached maximum at 8 or 10 hours, then decreased at 12 hours after LPS injection at the dose of 1.5μg/g. LPS injection resulted in the hepatic inflammation.Conclusion:The mice β-defensin3 gene expression were up-regulated in liver in a time-and dose-dependent fashion during the systemic acute-phase response,and related to the hepatic inflammation.