摘要
目的 :探讨胃泌素对大鼠胃粘膜环氧合酶 (COX)及生长因子表达的影响。方法 :雄性SD大鼠皮下注射胃泌素 1μg/kg、10 μg/kg或 10 0 μg/kg,Westernblot和免疫组化检查胃粘膜COX - 1、COX - 2、肝细胞生长因子(HGF)和肝素结合表皮生长因子样生长因子 (HB -EGF)表达。评价胃泌素受体拮抗剂YM0 2 2对COX - 1、COX - 2、HGF和HB -EGF表达的影响。结果 :胃泌素剂量依赖性地增加大鼠胃粘膜COX - 2和HB -EGF表达 ,而对COX - 1和HGF表达无明显影响 ;YM0 2 2阻断胃泌素诱导的COX - 2和HB -EGF表达。结论 :胃泌素调节大鼠胃粘膜COX -2和HB -EGF蛋白表达 ,提示COX - 2和HB -EGF参与与胃泌素相关联的胃粘膜增生和胃癌等的发病过程。
AIM: To clarify the effects of gastrin on t he expression of cyclooxygenase (COX) and several growth factors in rat gastric mu cosa. METHODS: Male Sprague Dawley rats were fasted for 24 hours and s ubcutaneously injected with saline or gastrin 17 at doses of 1 μg/kg, 10 μg/kg and 100 μg/kg, respectively. The expression of COX-1, COX-2, heparin-binding e p idermal growth factor-like growth factor (HB-EGF) and hepatocyte growth factor ( HGF) in the gastric mucosa were examined using Western blotting and immunohistoc hemical staining. Effects of a potent gastrin receptor antagonist YM022 on the e xpression of COX-1, COX-2, HB-EGF and HGF in gastric mucosa were also evaluated. RESULTS: Gastrin dose-dependently increased the expression of C OX-2 and HB-EGF in rat gastric mucosa while the expression of COX-1 and HGF did not change significantly after treatment with gastrin. However, pretreatment wit h YM022 dose-dependently abolished the up-regulation of COX-2 and HB-EGF express ion induced by gastrin. CONCLUSIONS: This study demonstrates that gastrin up-regulates C OX-2 and HB-EGF expression in rat gastric mucosa, indicating that COX-2 and HB-E GF are involved in pathogenesis of the gastrin-related gastric mucosal hyperplas ia and carcinoma of stomach.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第2期271-275,共5页
Chinese Journal of Pathophysiology
基金
教育部留学回国人员科研启动基金 (No .92 4 7342 0 5 7)
江苏省卫生厅"135工程"医学重点人才基金 (苏卫科教 [2 0 0 3]19号 )资助
