异体角膜刺激引起的人外周血T细胞及其亚群CD25分子表达

Influence of allogeneic cornea on human peripheral blood T lymphocyte subtype in vitro

目的 :观察异体角膜体外对人外周血T细胞及其亚群CD2 5分子表达的影响。方法 :异体角膜与外周血淋巴细胞体外共同培养后 ,进行单克隆抗体标记和流式细胞分析。结果 :对照组T细胞CD2 5表达为 2 5. 2 % ;受角膜或沸波醇酯 (PDB)激活后T细胞CD2 5表达分别为 5 6 . 8%和 80 . 9% ;受角膜和沸波醇酯共同激活后T细胞CD2 5表达为 70 . 2 % ;受异体角膜激活后 ,CD4和CD8T淋巴细胞CD2 5表达分别为 6 7 .3%和 5 2 . 3%。结论 :异体角膜组织体外能够刺激人外周T细胞CD2 5表达和T细胞活化 ,CD4T细胞比CD8T细胞活化更明显。

AIM: To observe the immunoregulation of all ogeneic cornea on the human peripheral blood T lymphocytes in vitro. METHODS: After Co-culture of human peripheral blood lymphocytes and allogeneic cornea in vitro, T lymphocytes were labeled by monoclonal ant ibody, and analyzed by fluorescent activated cell sorter (FACS). RESULTS: CD25 expression on T lymphocytes in control was 25.2%, after stimulated by the allogeneic cornea or PDB, CD25 expression on T lymphocy tes was 56.8% and 80.9%, respectively. After stimulated by the allogeneic corn ea, CD25 expression on CD 4+ or CD 8+ T lymphocytes were 67.3% and 52.3% , respectively. CONCLUSION: Allageneic cornea stimulates CD25 expression on huma n peripheral blood T lymphocytes, and the CD25 expression on CD 4+ T lymphocy tes is more prominent than CD 8+ T lymphocytes.