肉苁蓉对败血症大鼠胸腺细胞的保护作用

The protective effect of cistanche deserticola Y.C Ma . on thymocytes in septic rats

目的 :探讨肉苁蓉提取物对败血症休克大鼠胸腺细胞凋亡的影响及其可能机制。方法 :盲肠结扎穿孔术 (CLP)造成大鼠败血症休克。防治组术前 14d开始给药 (1 2 5g/kg) ,分别于术后 12h、2 4h取材。流式细胞仪检测细胞凋亡率和线粒体膜电位 ,分光光度法测定细胞线粒体ATP酶活力。结果预先给予肉苁蓉提取物的大鼠 ,行CLP术后 12、2 4h胸腺细胞凋亡率均低于模型组 (P <0 . 0 1) ,线粒体膜电位和ATP酶活力则显著上升 (P <0 . 0 1)。结论 :肉苁蓉提取物对败血症休克时胸腺细胞有保护作用 ,其机制可能是提高ATP酶活力 ,维持了细胞线粒体膜电位。

AIM: To study the protective effect of ci stanche deserticola Y. C Ma. on thymocytes in septic rats. METHODS: Cecum ligation perforation (CLP) was used to induce sep sis. Treatment group was treated with cistanche deserticola Y. C Ma. (1.25 g·kg -1·d -1, ig) for 14 days before CLP. Animals were killed 12 h or 24 h after CLP an d thmocyte s were collected. The ratio of thmocyte apoptosis and mitochondrial membrane pot ential were determined by the flow cytometry. The ATP activity was detected by s pectrophotography. RESULTS: The rate of thmocyte apoptosis significantly increased 12 h after CLP. The ATP activity decreased 24 h after CLP was significant. The extract of desert living cistanche effectively repressed the apoptosis of th ymocytes and maintained the mitochondrial membrane potential. CONCLUSIONS: The extract of cistanche deserticola Y. C Ma. p rotects thymocytes against apoptosis induced by sepsis. Maintaining of mitochond rial membrane potential may be the protective mechanism.