爱知结球白菜组培再生株与实生株的性状比较

Comparison of some characters between in vitro regenerated plants and seedling plants of Brassica campestris ssp. pekinensis ‘Aichi Hakusai’

对日本引进的爱知结球白菜〔Brassicacampestrisssp. pekinensis‘AichiHakusai’〕的组培再生株从染色体数目、形态特征及生化特性方面与实生株进行了比较。结果表明,再生株染色体数目与实生株相同,均为 2n=20;再生株在移栽初期长势不齐, 1个月后,生长趋于一致,形态指标均显著高于相同条件下栽培的实生株;两者叶片可溶性糖和可溶性蛋白质含量的变化趋势相似;可溶性蛋白质含量在移栽初期高,碳氮比 (C/N)小,代谢类型主要是氮素代谢;随着生长发育的进行,叶面积增加,碳水化合物代谢加强,其可溶性糖含量增加,C/N比逐渐上升。说明爱知结球白菜的组培再生株在所测定的性状上保持了实生株的遗传稳定性。

Brassica campestris ssp. pekinensis ‘Aichi Hakusai’ was introduced from Japan. Comparison of chromosome number, some morphological and biochemical characters was made between in vitro regenerated plants and seedling plants of ‘Aichi Hakusai’. The results indicated that the chromosome numbers of in vitro regenerated plants and seedling plants were the same (2n = 20). The growth of regenerated plants was not very uniform at the early stage after transplanting. One month after transplanting, the growth of regenerated plants became uniform, and their morphological indexes were significantly higher than those of seedling plants under the same cultivating conditions. The change trends of soluble sugar and soluble protein contents in leaves were similar between regenerated plants and seedling plants. At the early stage after transplanting, soluble protein content was increased, carbon-nitrogen ratio (C/N) was low, and metabolic type was mainly nitrogen metabolism. As growth and development of plants proceeded, carbohydrate metabolism was enhanced with the increase of leaf area, resulting in the increase of both soluble sugar contents and C/N ratios. The results show that regenerated plants of ‘Aichi Hakusai’ retain the genetic stability of the seedling plants in the characters.